The Effects Of Acorus Tatarinowii On Serum Brain-Derived Neurotrophic Factor And Vascular Endothelial Growth Factor In Rat Model With Post-Stroke Depression

Objective:To observe the healing Acorus tatarinowii behavior of poststroke depression rats and the effects of serum brain neural impact factor(BDNF)source,the expression of vascular endothelial growth factor(VEGF),discuss the role of traditional Chinese medicine in anti depression after stroke and serum BNDF,VEGF level correlation with depression after stroke.Methods:A total of 40 adult male SD rats were classified into sham operation group,depression group,PSD group and Chinese pharmacokinetic acorus prevention group,each group of 10.Reference of sun-style method for healthy male rats with abdominal cavity anesthesia,the neck skin,disinfection,cut,blunt isolated unilateral carotid artery,with incomplete unilateral carotid artery ligation of line 9,suture.A model of post-stroke depression rats was established.The prevention group of acorus acorus was found in 7d after surgery,CUMS,and was given water extract of acorus acorus [10g/Kg.d].For preparation of PSD rat gastric once a day the observation group rats spontaneous behavior change,the wilderness test using sucrose water consumption and weight change to assess the change of behavior in rats.After 21 days,serum BDNF and VEGF levels were measured by immunoassay(ELISA).SPSS21.0 statistical analysis software was used for statistical processing,and standard deviation()was used for the statistical results of measurement data.All calibration values should be checked before the statistics,and then the corresponding statistical methods are selected.The variance analysis method of One Way ANOVA was used to compare the data between multiple groups.The least significant method was selected between groups,namely LSD method.Statistical results were considered statistically significant with P < 0.05.Results:Compared with control group,model group showed weight growth slows,the stone calamus prevention group weight increased significantly,respectively(264.22±19.36)g vs(189.17±19.32)g g vs(195.66±11.57)vs(239.19±18.31)g g,including depression and PSD group statistically significant differences in the control group(P < 0.05),to prevent the PSD group difference was statistically significant(P < 0.05);Compared with control group,model group rats significantly lower sucrose water consumption,loss of appetite,including prevention group rats sucrose water consumption increase gradually,respectively(5.32±0.96)vs g / 100 g(3.25±0.92)vs g / 100 g(4.01±0.57)vs g / 100 g(6.99±0.78)g / 100 g,depression and PSD group statistically significant differences in the control group(P < 0.05),the prevention of the PSD group difference was statistically significant(P < 0.05);Groups of rats OFT horizontal movement distance: the changes of the levels of model group rats OFT movement distance were significantly lower than the control group,the stone calamus OFT horizontal movement distance of a rat prevention group was obviously higher than that of PSD group,respectively(4175.25±2012.92)cm / 5 min vs(3404.01±077.57)cm / 5 min vs(8818.32±1101.96)cm / 5 min;(8991.96±2177.78)cm/5min vs(3404.01±1077.57)cm/5min;The average concentration of BDNF in the hippocampus of rats in the model group was lower than that in the sham operation group(4752.32±1561.06)pg/ml vs(1723.22 ±1223.96)pg/ml vs(3266.88±1381.47)pg/ml;The mean concentration of BDNF was higher than that of PSD group,respectively(3673.03±1967.03)pg/ml vs(3673.03±1967.03)pg/ml;The average concentration of VEGF in the hippocampus of rats in the model group was lower than that in the sham operation group(76.01±13.92)pg/ml vs(51.09± 14.58)pg/ml vs(63.32±12.95)pg/ml.The concentration values of acorus were higher than that of PSD group(63.99±10.78)pg/ml vs(51.09±14.58)pg/ml.Conclusion:The level of brain-derived neurotrophic factor(BDNF)can be used as a correlation index of depression,and its concentration level is correlated with depression.Vascular endothelial growth factor(VEGF)concentration level was correlated with depression level,but it was not a relevant index to evaluate the degree of depression.It has the effect of anti-depressant,especially after ischemic stroke.