Clinical Value Of Hypersensitive HBV DNA In Chronic Hepatitis B Viral Hepatitis

ObjectivesDue to the limitation of conventional detective methods,fewer studies were conducted of the levels of Hepatitis B surface antigen(HBsAg),hepatitis B e antigen(HBeAg),glutamic-pyruvic transaminase(ALT)、Glutamyl transpeptidase(GGT)、albumin、glucose(GLU)、alkaline phosphatase(ALP)、triglyceride(TG)、total cholesterol(TC)、prothrombin time(PT)、activated partial thromboplastin time(APTT)in chronic hepatitis B patients with low HBV load.Therefore,the aim of study was to measure the viral replication index of hepatitis B virus(HBVDNA),combine the clinical characteristics to investigate the relationship and difference of HBsAg,HBeAg,ALT,AST,GLU,LDL,HDL,TG,TC,ALP,GGT,PT,APTT in CHB patients.These results will provide the theoretical basis for diagnosis and treatment of CHB patients.MethodsTotal of 160 serum samples of chronic hepatitis B patients(HBVDNA<500IU/ml)were included from December 2016 to June 2017.The general clinical features of all the subjects were recorded,and the serum levels of HBs Ag and HBeAg were measured using Abbott Laboratories i2000.Automatic biochemical analyzer was used to detect the level of ALT,AST,GGT,ALP,TG,TC,LDL,GLU,albumin.Automatic nucleic acid extraction and fluorescence analysis system was used to detect the levels of HBVDNA.The results such as gender,age,HBsAg、HBeAg、HBVDNA、ALT、GGT、ALP、TG、TC、LDL、HDLwere statistically analyzed.Results1.65 cases of all the HBV infected CHB patients were divided into the positive group(HBVDNA: 20~500IU/ml),in which the males account for 63%,and the samples of ages between 31~60 account for 72%.95 cases of all the HBV infected CHB patients were divided into the control group(HBVDNA<20IU/ml),in which the males account for 59%,and the samples of ages between 31~60 account for 65%.2.The differences of HBsAg,HBeAg,ALT,AST,GLU,LDL,HDL,TG,TC,ALP,GGT,PT,APTT in each group were analyzed,Difference of TG and ALT in the positivegroup and the control group was significant different(P < 0.05),no significant different of AST,GLU,LDL,HDL,TC,ALP,GGT,PT,APTT were observed in the positive group and the control group(P > 0.05).3.The differences of HBs Ag and HBeAg in each group were analyzed,.and no significant differences were observed in the positive group and the control group(P >0.05).4.65 cases of all the HBV infected CHB patients in the positive group(HBVDNA:20~500IU/ml),were divided into the positive group(HBe Ag>1.0COI)and the control group(HBeAg<1.0COI),in which the differences of HBsAg,HBe Ag,ALT,AST,GLU,LDL,HDL,TG,TC,ALP,GGT,PT,APTT in each group were analyzed,Difference of ALT in the positive group and the control group was significant different(P < 0.05),no significant different of AST,GLU,LDL,HDL,TC,TG,ALP,GGT,PT,APTT were observedin the positive group and the control group(P > 0.05).5.95 cases of all the HBV infected CHB patients in the positive group(HBVDNA<20IU/ml),were divided into the positive group(HBe Ag>1.0COI)and the control group(HBeAg<1.0COI),in which the differences of HBsAg,HBeAg,ALT,AST,GLU,LDL,HDL,TG,TC,ALP,GGT,PT,APTT in each group were analyzed,no significant different of age,gender,ALT,AST,GLU,LDL,HDL,TC,TG,ALP,GGT,PT,APTT were observedin the positive group and the control group(P > 0.05).ConclusionsFor routine detection of HBVDNA < 500 IU/ml,the virus replication still existed in a low level although the HBe Ag levels were negative.Patients with low viral load still have the increased risk of cirrhosis and hepatocellular carcinoma.More sensitive HBVDNA quantitative determination will contribute to find occult HBV infection.More accurate detection for the HBVDNA should be made,combined with patient virology indexes and liver damage index,to further evaluate the patient condition and get the better disease outcomes.