Effects Of Sleep Deprivation On The Ability Of Learning And Memorizing And The Oxidative Stress And Inflammation In Mice Brains And Livers

Objective: Using the modifid multiple platrorm water environmental method to establish sleep deprived mice model,in order to investigate the effects of sleep deprivation(SD)on the ability of learning and memorizing and the function of liver and brain in mouse,and to explore the possible mechanism.Methods: Twenty C57BL/6J female mice at the age of 4 weeks were randomly divided into normal control group(n=10)and SD group(n=10),mice in SD group were treated with MMP-WM method,the general condition and body weight of two groups were observed and recorded during sleep deprivation.Then forty-five days after SD,the mice were observed for the changes of body weight,and the percentage of weight gain was detected in each group.Learning and memorizing abilities were measured by Morris Water Maze Test.Liver,spleen and kidney wet weight index were detected in each group.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)in the serum were detected by ELISA.The activities of superoxide dismutase(SOD)and contents of reduced glutathione(GSH)were measured by microplate assay.The protein levels of TNF-α,P53,Bcl-2-associated X protein(BAX)and B-cell lymphoma-2(BCL-2)were measured by Western blot.The expression of Ki-67 was analyzed by immunohistochemical technique.Results: Sleep deprived model in mice was established successfully.There was no significant difference in body weight between the two groups before sleep deprivation,but forty-five days after SD,compared with the normal control group,the mice in SD had a relatively slow body weight gain,a reduced spatial learning and memorizing capacities,escape latency and swimming distance in the sleep deprivation group were significantly higher(P<0.05),the numbers to cross the original platform and the percentage of time spent in the original target quadrant were significantly lower(P<0.05).An increased liver and kidney wet weight index,and a decreased spleen wet weight index,differences are statistically significant(P all <0.05).The levels of TNF-α,IL-6 and IL-1β were higher in SD group than those in the normal control group(P<0.05);the activities of SOD and contents of GSH in hippocampus and livers in SD group lowered significantly,compared with those in the normal control group(P<0.05).Compared with the normal control group,the expression level of TNF-α,P53 and BAX in hippocampus and livers increased significantly(P<0.05),while the expression level of BCL-2 decreased significantly(P<0.05),and the positive expression of Ki-67 in hippocampus decreased significantly(P<0.05).Conclusion: SD affects the body weight,spatial learning and memory ability and organ index,induces the oxidative stress of brains and livers in mice and elevates the concentration of inflammatory cytokines in the brain and serum,and its mechanism may be related to the regulation of DNA damage through cell apoptosis.This provides a certain theoretical basis for the impact of sleep-deprived on the body.