MTHFR Gene Polymorphism In Young Patients With Chronic Atrophic Gastritis In Shaanxi

ObjectiveTo investigate the relationship between genetic polymorphisms of Methylenetetrahydro folate Reductase(MTHFR)and chronic atrophic gastritis(CAG)in young patients of Shaanxi Province.MethodsA total of 50 patients diagnosed with CAG at the First Affiliated Hospital of Xi’an Medical University from March 2017 to December 2017 were collected.During the same period,50 volunteers were recruited into the healthy control group at the First Affiliated Hospital of Xi’an Medical University.In this study,paramagnetic microparticle chemiluminescence immunoassay and PCR-Taqman probe technique were used to detect serum and red cell folate concentrations and the type of MTHFR gene in all subjects.The case-control study was used to compare the differences in the serum folate,red cell folate,and the frequencies of MTHFR gene polymorphisms in the CAG and control.The scores of clinical symptoms,endoscopic mucosa and histopathology in the CAG group were compared with the correlations of serum folic acid,red cell folate and MTHFR gene distribution frequencies with the severity of gastritis.And compare the differences in the distribution of different genotypes and the concentration of red cell folate.Results(1)The red cell folate concentrations in the CAG case group and the healthy control group were 343.04±54.03ng/mL and 368.40±66.60ng/mL,there were statistically significant differences(P<0.05);the serum folate concentrations were 4.97±1.54ng/mL,5.69±1.75ng/mL,there were statistically significant differences(P<0.05).(2)The frequencies of MTHFR C677 T and MTHFR A1298 C genes in case group and control group were all consistent with Hardy-Weinberg’s law of genetic equilibrium.The frequencies of CC,CT and TT genes of MTHFR C677 T in CAG case group were 28%,36%,and 36%,respectively,the frequencies of CC,CT and TT genotypes of MTHFR C677 T in the healthy control group were 44%,42%,and 14%,the frequencies of the 677 C allele andthe 677 T allele in the case group were 46% and 54%,the frequencies of the 677 C allele and the 677 T allele in the control group were 65% and 35%,there were significant differences in the frequency distribution of genotypes and C,T alleles of MTHFR C677 T between the two groups(P<0.05).The frequencies of AA,AC and CC genes of MTHFR A1298 C in CAG case group were 44%,44%,and 12%,the frequencies of AA,AC and CC genes of MTHFR A1298 C in healthy control group were 26%,40%,and 34%,the frequencies of the 1298 A allele and the 1298 C allele in the case group were 66% and 34%,the frequencies of the1298 A allele and the 1298 C allele in the control group were 46% and 64%,there were significant differences in the frequency distribution of genotypes and A,C alleles of MTHFR A1298 C between the two groups(P<0.05).(3)By Spearman correlation analysis,there was no correlation between clinical symptoms and red cell folate concentration,serum folate concentration in CAG group(P>0.05);The scores of gastroscopic were negatively correlated with red cell folate concentrations(P<0.05),and no correlation with serum folate concentrations(P>0.05);the scores of histopathological(Hp infections,activity)were no correlation with red cell folate concentration,serum folate concentration(P>0.05);the scores of histopathological(chronic inflammation,atrophy,intestinal metaplasia)was negatively correlated with red cell folate concentration(P<0.05),but not correlated with serum folate concentration(P>0.05).(4)Logistic regression analysis showed that compared with 677 CC,the risk of CAG of677 CT increased 1.34 times,the difference was not statistically significant(P>0.05);the risk of CAG of 677 TT increased 4.04 times,the difference was statistically significant(P<0.05).Compared with the 677 C allele,the risk of CAG of the 677 T allele was 2.18 times higher,with a statistically significant difference(P<0.05).The 677 T allele is a risk factor for CAG.For MTHFR A1298 C,compared with type 1298 AA,the risk of CAG in type 1298 AC decreased by 0.65-fold,and the difference was not statistically significant(P>0.05),the risk of CAG in type 1298 CC decreased 0.21-fold,the difference was statistically significant(P<0.05).Compared with the 1298 A allele,the 1298 C allele had a 0.43-fold reduction in CAG risk,with a statistically significant difference(P<0.05).The 1298 C allele is a protective factor for CAG.(5)The red cell folate concentration in the CAG case group and the healthy control group were different within the group(P<0.05),there was no statistically significant difference between the two groups(P>0.05).In the CAG case group and the healthy control group,the red cell folic concentrations of MTHFR 677 CC and 677 CT,677TT werestatistically significant(P<0.05);the difference in red cell folic concentrations between MTHFR 677 CT and 677 TT was not statistically significant(P>0.05).There was a significant difference in the concentration of red cell folic between MTHFR 1298 CC and 1298 AA,1298AC(P<0.05),there was no significant difference in the concentration of red cell folic between MTHFR 1298 AA and 1298AC(P>0.05).Conclusions(1)Serum folic acid and red cell folate concentrations in the CAG case group were significantly lower than those in the healthy control group.Detecting red blood cell folate is more valuable than detecting serum folate in the assessment of the severity of CAG.(2)CAG is associated with MTHFR gene polymorphism,and MTHFR 677 T allele and the 1298 A allele were CAG risk factors.