The Neuroprotective Effect Of Cannabidiol On Spinal Cord Injury Mice

Object:To investigate the inhibition of inflammation by cannabidiol in a mouse model of spinal cord injury and study its mechanism.Methords:24 male C57B1/6 mice were randomized into 3 groups:shamoperation group,control group and medication group.We established an spinal cord injury model by clip method,the mice in sham-operate group and control group were administered with a mixture of 1%of tween 80 and sterile saline(0.25ml/10g)and medication group were treated with cannabidiol(30mg/kg;was dissolved in a mixture of 1%of Tween 80 and sterile saline(1.2mg/ml))by intraperitioneal injection at 1h、12h、24h、36h、48h、60h after operation.72h after operation,BMS test was used to evaluate the neurological score,the expression levels of HMGB1 and TNF-αin spinal cord tissue were assayed by ELISA,the expression levels of Caspase-3 were assayed by Western-Blot and Immunofluorescence.Rsult:The BMS test showed that medication group had a higher score than the control group(P<0.05).The expression levels of HMGB1,caspase-3 and TNF-α in medication group were significant reduced as control group P<0.05),but significant increased than the sham-operation group(P<0.05).The expression levels of Caspase-3 in medication group were significant reduced as control group P<0.05),but significant increased than the sham-operation group(P<0.05).Conclusions:cannabidiol can inhibit inflammation in a mouse model of spinal cord injury via inhibiting the expression of HMGB1,and can inhibit Apoptosis in a mouse model of spinal cord injury.