Study On Toxicity Of Acifluorfen To Liver,Kidney Tissure And Blood System

In twenty-first century,the "high efficiency" "low toxicity" and "security" is the theme of new herbicides.Acifluorfen is a new herbicide to plant protoporphyrinogen oxidase as a target,this kind of herbicide mainly through the active site of the enzyme protoporphyrinogen IX competition to inhibit protoporphyrinogen oxidase activity protoporphyrinogen oxidase inhibitor.Protoporphyrin oxidase inhibitor is the most frequently applied compound in recent years,and it is also one of the most active research fields of chemical pesticides.In animal,plant and fungal organisms,protoporphyrinogen oxidase plays an important role in heme or chlorophyll synthesis.Porphyrin is a carcinogen,which has photochemical cytotoxicity that it can produce single state oxygen under the condition of oxygen.Excessive single oxygen can cause oxidative damage of body tissue.In addition,heme is a functional group of many important proteins in the organism.At present,there are few studies on the toxicity of acifluorfen.The toxicity of acifluorfen was investigated by liver,kidney and blood test in this topic.In this experiment,35 Balb/c mice were randomly divided into five groups,including one blank control groups,three different dose exposure groups and a vitamin E protection group.The exposure group was divided into 0.1 mg/kg,5 mg/kg,250 mg/kg three dose levels,and the time was 14 days.The bodyweight,organ coefficient and liver and kidney histopathological slices after exposure to acifluorfen were tested to evaluate the effects of acifluorfen on liver and kidney tissue in mice.The indexes of oxidative stress in liver and kidney tissue were detected(reactive oxygen species(ROS),glutathione(GSH)and malondialdehyde(MDA),DNA damage index 8-hydroxydeoxyguanosine(8-OHdG)and related indexes of apoptosis(expression and cytochrome TNF-α content,caspase-3 protein,blood routine examinationtest(hemoglobin,red blood cells,white blood cells,lymphocytes and neutrophils)and blood biochemical index,including glucose metabolism indexes(blood sugar),regulating lipid metabolism indexes(blood lipids,cholesterol),protein metabolic regulation index(total protein,albumin,globulin),hepatobiliary excretion index(intake of total bilirubin and direct bilirubin and total bile acid),liver cell membrane integrity index(ALT and AST)and renal function related blood biochemical indexes(blood urea nitrogen and creatinine content to explore the possible mechanism of the production of acifluorfen.The protective effect and protective mechanism of vitamin E on liver and kidney tissue after exposure to acifluorfen was studied by adding vitamin E as a protective agent.The experimental results show the group of 250 mg/kg/day mice liver cell rupture,nuclear condensation,hydrolysis,cytoplasmic vacuolation,bright and empty,severe necrosis of local cell injury,and kidney tissue also appeared the number of glomeruli reduce,renal corpuscle hyperplasia and cystic wall glomus,unclear symptoms.Compared with the control group,the organ coefficient,ROS,MDA,8-OHdG and caspase-3 protein contents in the liver tissues of mice were significantly increased when the dosage was 250 mg/kg(P<0.05),and the contents of cytochrome C and GSH decreased significantly(P<0.05).Similarly,the organ coefficient,ROS,MDA,8-OHdG,TNF-a and caspase-3 protein contents of the same kidney tissues increased significantly(P<0.05),and the contents of cytochrome C and GSH decreased significantly(P<0.05).The content of hemoglobin and the number of red blood cells in the blood of mice decreased significantly(P<0.05).The blood sugar,albumin,albumin/total protein decreased significantly.Total bilirubin,direct bilirubin and total bile acid were significantly increased.The content of AST,ALT and ALP were significantly increased,blood urea nitrogen and creatinine content increased significantly.Vitamin E(100 mg/kg/day)can reduce oxidative stress,thus making the acifluorfen damage on mice liver and kidney tissue improved to a certain extent.