| Objective:A rat model of sick sinus syndrome was established by sodium hydroxide intervention.On the basis of establishing a successful rat model,a randomized controlled experimental method was adopted to observe the changes of body temperature,respiratory rate,heart rate and heart rhythm of rats after 14 days of modeling before,after model building and intragastric Fuxin pulse,and to explore the effect of compound heart pulse prescription on sick sinus syndrome model rats.Methods:1.The rat model of sick sinus syndrome was established by the interventional method of sodium hydroxide.The rats after the intraperitoneal injection of-chloral hydrate were fixed on the rat,and the limbs were prepared to connect the electrocardiogram machine.The electrode catheter was inserted vertically into the second intercostal spaces of the right border of the sternum to observe the change of the V1 waveform on the electrocardiograph,and to find the location of the sinoatrial node in the rat.After accurate positioning,10%sodium hydroxide solution was injected through a self-made electrode catheter using a micro syringe.The heart rate of 2 h in rats was observed continuously.The rats with heart rate drop of 2 h after modeling were successful models of sick sinus syndrome.2.The effect of Fuxin Mai Recipe on the body temperature,respiratory rate,heart rate and heart rhythm of the model rats:40 rats were selected and randomly divided into the high dose group of Fuxin Mai Fang,the dose group of Fuxin Mai Fang,the low dose group of Fuxin Mai Fang and the control group,with a total of 4 groups of 10 rats in each group.The rats in the model group were administrated with gavage for third days after modeling,and continued for 14 days.The body temperature,respiratory rate,heart rate and cardiac rhythm of 4 groups were observed before modeling,after modeling and 14 days after administration.Results:1.The rat model of sick sinus syndrome was established by sodium hydroxide intervention:the heart rate of 2 h in the sick sinus rat model established by sodium hydroxide intervention was about 36%.The heart rate of 5 min,10 min,30 min,60 min and 120 min after the modeling was significantly lower than that before the model,and there was a significant difference(?P<0.001),and the success of the model was successful.The rate is 90%.2.The effect of Fuxin Mai Recipe on body temperature,respiratory rate,heart rate and heart rhythm of the model rats:the 4 groups of rats were compared with the model before the model,and there was a significant diff'erence between the temperature,the frequency of respiration and the decline of heart rate(?P<0.001).After the administration,the body temperature of the high(?P<0.001 or ? P<0.05),middle and low dose group was compared with that after the model.The respiratory rate decreased(?P<0.01 or ? P<0.05)),the heart rate increased(? P<0.001 or ? P<0.05),and the sinus node function score decreased(? P<0.05)in the high,middle and low dose group.After administration,the body temperature and heart rate in the high dose group were significantly better than those in the middle and low dose group and the control group,with statistical significance(?P<0.001 or?P<0.05).Conclusion:1.Sodium hydroxide intervention can successfully establish the rat model of sick sinus syndrome.The model rats established by this method are more accurate and the success rate of modeling is higher.2.Fuxin Mai recipe can increase the body temperature,reduce the respiratory rate and increase the heart rate in the sick sinus syndrome model rats,and reduce the arrhythmia caused by the disease.There was a dose-dependent relationship between body temperature and heart rate. |
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