Expression Of KIF20A Correlates With Prognosis In Gastric Cancer

Purpose To detect the expression of kinesin family member 20A(KIF20A)in gastric cancer and adjacent normal mucosal tissue,and to explore the relationship between KIF20 A expression and clinicopathological characteristics in gastric cancer(GC).Methods m RNA and protein levels of KIF20 A were detected by quantitative real-time PCR(q RT-PCR)and Western blot in 38 pairs of fresh GC and corresponding normal specimens.After lentivirus-mediated KIF20 A for GC cell lines,cell viability and colony formation assay were carried out to evaluate the effects of KIF20 A on GC in vitro.Immunohistochemistry assay based on a tissue microarray(TMA)consisting of 146 cases was performed to evaluate the prognosis value of KIF20 A.Results We found that KIF20 A expression level is increased in GC(P<0.05).Up-and down-regulating KIF20 A markedly affected cell proliferation in vitro.TMA showed that percentage of KIF20A(+)is significantly higher in GC tissues.Pearson χ2 test method indicated the close relationship between KIF20 A and histological grade(P=0.036).Besides,survival analysis showed that median survival for patients with KIF20A(+)was 40.2 months and for patients with KIF20A(-)was 48.9 months.Therefore,KIF20 A should be an independent prognostic factor.Conclusions 1)KIF20A expression is upregulated at both the transcriptional and translational levels in GC;2)KIF20A promote cell proliferation in vitro;3)Histological grade is poorer in GC patients with KIF20A(+);4)KIF20A is an independent prognostic factor for GC.