Effects Of Dietary Copper Levels On Intestinal Morphology And Development In Pigs And Mice

This article aims to study the effect of dietary copper levels on the intestinal morphology and development of pigs and mice.In the experiment 1,ninety(Duroc × Landrace × Yorkshire)aged 21-day-old piglets were randomly divided into five groups.Divided into two feeding phases,phase one: 1 to 21 days,phase two: 22 to 163 days.Phase one is fed with 25mg/kg,50mg/kg,75mg/kg,100mg/kg,and 125mg/kg copper content,and phase two is fed the same copper-containing feed.By detecting the swine intestinal morphology,intestinal development and in vitro copper sulfate pentahydrate on porcine organoid activity,suggesting that dietary copper may affect pig intestinal morphology and development by regulating pig intestinal stem cells.The main research results are:(1)With the increase of copper content in the diet during weaning,the villi width of weaned piglets decreased significantly(P < 0.05),the number of Ki-67 positive cells was significantly increased(P < 0.01).the villi height of finishing pigs increased significantly(P < 0.01).(2)Compared with the control group,10μmol/LCuSO4.5H2 O can significantly improve the budding efficiency of organoids(P < 0.05),while 100μmol/LCuSO4.5H2 O can significantly inhibit the organoid budding efficiency,crypt depth and each organoid crypts(P < 0.05).In the experiment 2,Twenty-three wild-type mice with the same genetic background at the age of eighteen days were randomly divided into two treatment groups。Divided into two feeding phases,phase 1: 1 to 14 days and phase 2: 15 to 56 days.In the first phase,6 mg/kg and 125 mg/kg copper were added to the diet,and in the second phase,the same copper-containing feed was fed.By testing the growth performance,intestinal morphology,intestinal development and jejunal organoid activity of weaned and adult mice showed that high levels of dietary copper can inhibit the growth of weaned mice,and dietary copper may affect mice intestinal development by regulating mice intestinal stem cells.The main research results are:(1)High levels of dietary copper significantly reduced the weight of weaned mice and the ratio of feed to meat was significantly increased.The ratio of feed to meat of adult mice was significantly decreased.(2)High levels of dietary copper significantly reduced the small intestine weight and large intestine length in weaned mice(P < 0.05),and significantly increased the relative length of the small intestine,the relative weight of the small intestine,the relative length of the large intestine,and the relative weight of the large intestine(P < 0.01).(3)High levels of dietary copper significantly increased the relative weight of the small intestine in adult mice(P < 0.01),and the relative length of the large intestine also showed an increasing trend(P = 0.075).(4)High levels of dietary copper in the diet can significantly increase the rate of organoid formation in weaned mice(P = 0.000),and have an increasing trend in the organoids budding efficiency of adult mice(P = 0.06).In summary,dietary copper during weaning may affect the intestinal morphology and development of weaned and finishing pigs by affecting the activity of pig intestinal stem cells.For mice,dietary copper during weaning may also affect intestinal development in weaned and adult mice by affecting intestinal stem cell activity.This provides a certain theoretical basis for the copper in the diet may affect the intestinal morphology and development by affecting the intestinal stem cells.