Preparation And Properties Of Gelatin Based Ce?no₃?₃ Antibacterial Fiber Film With Tfea/dw As Co-solvent

Gelatin-based electrospun antibacterial fiber film can be widely used in the fields of biomedicine,energy and food industries because of its excellent physical and chemical properties and biocompatibility.The gelatin solution system has poor stability during the spinning process,and the research on gelatin electrospinning has been carried out for several decades.The researchers found that formic acid,hexafluoroisopropanol(HFIP),2,2,2-trifluoroethanol(TFEA)and acetic acid solvent systems were suitable for the electrostatic spinning of gelatin.However,the organic solvent remaining in the fiber film has an influence on the future development of the application.In this paper,TFEA and deionized water(DW)were used as co-solvents,gelatin was used as the matrix material,and Ce(NO3)3 was used as antibacterial agent.In combination with the rapid solubility of gelatin in DW and the low foaming and stability in TFEA,a uniform,bubble-free gelatin-based electrospinning solution is rapidly prepared.The influence of rheology of gelatin-based electrospinning solution on gelatin-based electrospun fiber film was studied.The gelatin-based Ce(NO3)3 fiber films were prepared by electrospinning and its antibacterial properties and other physical and chemical properties were analyzed and characterized.Firstly,the effects of different TFEA/DW ratio,gelatin content and Ce(NO3)3 content on the rheological properties of the spinning solution were studied.The regularity was calculated and the appropriate spinning ratio was obtained according to the empirical formula.The antibacterial performance test was carried out on the cerium nitrate solution and the cerium nitrate-containing gelatin-based spinning solution to determine the antibacterial activity of the cerium nitrate bulk and the antibacterial activity of cerium nitrate in the gelatin-based spinning solution.Subsequently,gelatin-based yttrium nitrate fiber film was designed and tested for its antibacterial properties,surface morphology,mechanical properties,hydrophobicity and thermal stability,and the interaction between Ce(NO3)3 and gelatin molecular chains in the fiber film was investigated.It provides an empirical basis for the development of gelatin-based cerium nitrate fiber film in the future electrospiuning technology and lays a foundation for future industrial development.Studies have shown that at room temperature,with TFEA/DW as a co-solvent,when TFEA accounts for 30%of the solvent,gelatin can remain in a liquid state for a long time in a certain concentration range.The ratio of TFEA/DW to 3/7 is the co-solvent ratio of gelatin-based electrospinning,which can keep the gelatin-based spinning solution stable spinning while reducing the potential toxic effects caused by TFEA.According to the rheological effect of different gelatin concentration on the gelatin-based spinning solution,a uniform non-bead fiber can be obtained when the gelatin concentration is above 12.5%.The gelatin-based Ce(NO3)3 fiber film obtained has a certain inhibition on the growth of Gram-negative bacteria(E.coli.)and Gram-positive bacteria(S.aureus).At the same time,the gelatin-based Ce(NO3)3 fiber film has faster water absorption,good thermal stability and certain mechanical properties after crosslinking.