| Singlet oxygen is believed to be the major biotoxic substance in photodynamic therapy(PDT).Quantitative measurement of 1O2 production and its distribution by direct detection the near-infrared luminescence of singlet oxygen(1O2)at 1270 nm have become the frontier hotspot of dose evaluation of PDT.In this thesis,1O2 luminescence detection system with gated photomultiplier tube(PMT)was proposed,and the switch signal of PMT and its response relationship with different pulsed light sources were analyzed.Then a time-resolved 1O2 spectral data analysis system based on MATLAB GUI has been designed,which provides a more efficient and more convenient way for analyzing and processing large quantities of time-resolved 1O2 spectral data.Finally,we successfully built the 1O2 luminescence detection system with gated PMT,and on basis of optimizing the triggering time and threshold voltage of the detection system the 1O2 luminescence detection experiments of different photosensitizers were carried out.The obtained results indicate that the developed 1O2 luminescence detection system with gated PMT can detect 1O2 luminescence signal,which have the potential application for studies of PDT-1O2 dosimetry. |
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