The Mechanism Of Brain Cell Apoptosis And Tau Protein In Ischemic Stroke Rats

Ischemic stroke is a kind of common disease that seriously endangers human health and quality of life.It belongs to the category of "stroke" in traditional Chinese medicine.It is the first four stubborn diseases of "wind,tuberculosis,heave and diaphragm" in traditional Chinese medicine.Ischemic stroke can lead to cerebral blood supply disorder due to various factors,resulting in cerebral hypoxia and ischemic necrosis,showing a neurological impairment.Ischemic stroke has the characteristics of high incidence,high mortality and high disability.About 4 million 600 thousand people die from stroke in the world every year.The number of deaths in our country is about 1 million 600 thousand,and about 50%to 70%of the surviving patients have sequelae,which is the second killer of human being.Therefore,exploring the pathogenesis of ischemic stroke has a very important practical significance for prevention and early treatment of stroke.With the continuous study of pathophysiological mechanism of ischemic stroke,the relationship between apoptosis and tau protein in ischemic stroke has attracted more and more attention.Therefore,we should explore the research progress of apoptosis and tau protein in ischemic stroke,so as to provide theoretical support for better prevention and treatment of ischemic stroke.We refer to the relevant literature at home and abroad,take the ischemic stroke rat model as a breakthrough point,try to reveal the mechanism of apoptosis in cerebral tissue and the correlation with tau protein and p-tau protein in ischemic stroke,and provide a way for the new drug development and treatment of ischemic stroke.objectiveThe purpose of this study is to investigate the changes in the apoptosis of brain tissue and the expression of tau protein and p-tau protein in cerebral tissue of rats with ischemic stroke.By observing the expression of tau protein,p-tau protein and apoptosis related protein bcl-2,bax,and apoptosis of the brain tissue at different time points of ischemic stroke,the intervention nerve was studied.The aim of this study is to provide a theoretical basis for the new target of apoptosis,in order to provide some guidance for the clinical treatment of ischemic stroke.MethodThe rat model of middle cerebral artery occlusion MCAO was established by the thread emboli method.The right brain of the rat was embolized in this experiment,and the behavior changes of the rats were observed before and after the model of the rat model.The brain tissue of rats was regularly fixed,paraffin embedded and sliced.The morphological changes of brain tissue were observed by HE staining.The expression of tau protein in the brain tissue of rats was detected by immunohistochemistry.The changes of bax and bcl-2 content of cell withering protein and bcl-2 were detected by immunoblotting test(Western blot).The changes of tau protein and p-tau protein in rat brain tissue.SPSS20.0 statistical software was used for statistical analysis.The data were expressed in X±s,the data were all normal distribution,single factor analysis of variance(one-way ANOVA),and LSD method was used to compare the two methods,with P<0.05 as the difference was statistically significant.Result1.The identification of the MCAO model:the rats have different degrees of abnormal behavior after the middle cerebral artery embolism,such as the left claw cannot be fully stretched,the standing is unstable,and there is a circle or tail after the complete sobriety.2.the morphological changes of the brain tissue:under the optical microscope,the right cerebral cortex in the normal group and sham group showed that the right cerebral cortex was complete and clear,the cells arranged neatly,the cell nuclei were dyed deep,blue purple,and the cytoplasm was pink,and the cell structure of the brain tissue was normal.The brain tissue of group MCAO rats had no obvious pathological changes.After the model of 12h,the brain tissue was observed.A small number of neurons were swollen and coloured.The single round apoptotic body could be observed.After modeling 24h,the brain tissue was observed,the cell swelling was obvious,the cell nucleus was conspicuous and fractured,and the brain tissue was observed after the model of 48h.Cell swelling and degeneration,nuclear condensation and deep staining,apoptotic bodies were observed.3.tau protein results:the content of tau protein in brain tissue of rats was detected by Western blot method.The results showed that the expression of tau protein in the brain tissue of MCAO model rats increased(P<0.05,P<0.01)compared with the normal group rats.Compared with the normal group,the brain tissue tau protein of the rats in the sham operation group was higher than that of the normal group(P<0.05,P<0.01).The results of immunohistochemical staining of tau protein in the brain tissue of rats showed that the positive staining of tau protein was brown and the coloring position was mainly distributed in the cytoplasm,and the positive expression was mainly in the cortex of the cerebral cortex.In the normal group and the sham operation group,a small number of positive cells could be seen,and the number of positive cells began to rise after the hypoxic and ischemic 3 H in the rat brain,and the number of positive cells increased obviously after the hypoxia ischemia 12h in the rat brain tissue,and the number of positive cells reached the peak when the brain tissue was hypoxic and ischemic 24h in the rat brain,and the positive cells were positive when the rat brain tissue was hypoxic and ischemic 48h.The number of cells decreased slightly,but higher than that of normal group and sham operation group.4.bax protein results:the content of apoptotic protein bax was detected by Western blot method.Compared with the normal group,the expression of bax protein in the brain tissue of the MCAO model rats increased(P<0.05,P<0.01).5.bcl-2 protein results:the content of apoptotic protein bcl-2 was detected by Western blot method.Compared with the normal group,the expression of bcl-2 protein in the brain tissue of the MCAO model rats increased(P<0.05,P<0.01).6.p-tau protein results:the content of p-tau protein in brain tissue of rats was detected by Western blot method.Compared with the normal group,the expression of p-tau protein in the brain tissue of the MCAO model rats increased(P<0.05,P<0.01).Compared with the normal group,the rat brain tissue p-tau protein was higher than that of the normal group(P<0.05,P<0.01).conclusion1.The rats have different degrees of abnormal behavior after the middle cerebral artery embolism,such as the left claw can not extend completely,stand unstable,and follow the symptoms of circle or tail after complete sobriety,proving the model is successful.2.The ratio of apoptosis related factor bax and bcl-2 protein increased,and the ratio of them decreased,suggesting the initiation of apoptosis in brain tissue after ischemic stroke.3.The expression of tau protein in MCAO rats was positively correlated with the apoptosis related factor bax protein and bcl-2 protein,suggesting that the increase of tau protein was associated with the apoptosis of ischemic stroke cells.4.The expression of tau protein and p-tau protein in MCAO rats increased,and was associated with apoptosis of nerve cells,suggesting that tau protein and p-tau protein were involved in the apoptosis of ischemic stroke cells.