Effect Of Bushen Huoxue Recipe Combined With Microencapsulated Ovarian Granulosa Cell Transplantation On Serum FSH, LH And E2 In Ovariectomized Rats

Objective:To investigate the effects of Bushen Huoxue prescription and transplanted microencasulated granulosa cells on rats reproductive and endocrine systems.Methods:Total 60 SD female rats were randomly divided into six groups:the blank group（A group）,the model group（B group）,microencapsulated granulosa cells transplanted group（C group）,the low dose Bushen Huoxue prescription and microencapsulated granulosa cells transplanted group（ D group）,the middle dose Bushen Huoxue prescription and microencapsulated granulosa cells transplanted group（E group）,the high dose Bushen Huoxue prescription and microencapsulated granulosa cells transplanted group（ F group）.After 28 days treatment,the rats were sacrificed and taken material.Enzyme-linked immune sorbent assay（ELISA） was adopted to detect the concentrations of serum estradiol（E₂）,follicle stimulating hormone（FSH）,and luteinizing hormone（LH）.Results: B group weights and ovarian index less than other five groups, significant diffenrence（P<0.05）,F group ovarian index is higher than B group, significant diffenrence（P<0.05）.In D group,E group and F group weight increases,but the difference is not statistically significant（P>0.05）.After removal of the ovaries, B group E₂ level less than A group obviously（P<0.01）, whereas FSH and LH levels increased significantly（P<0.05）.Compare C group with B group, FSH and LH levels decreased, E₂ level increased, significant diffenrence（P<0.05）;FSH and E₂ level between A group and F group have no statistically difference（P>0.05）.FSH,LH,and E₂ level has difference between C group and F group（P<0.05）.Conclusion:Compared with simple transplantation of microencapsulated granulosa cell,Bushen Huoxue prescription and transplantation of microencapsulated granulosa cell in ovariectomized rats can better improve hormone secretion condition,provide a good prospect for Chinese medicine of clinical prevention and treatment of premature ovarian failure.