Changes In Intestinal Flora And Related Cytokines In Children With Cholestatic Liver Disease

PART ONETO DETECT HEALTHY INFANT GUT MICROFLORA AND TO ANALYZE THE INFLUENCE OF DELIVERY AND FEEDING MODES ON GUT MICROFLORAObjective:To detect infant gut microflora; investigate the changes in gut microflora of healthy infants; analyze the influence of delivery and feeding modes on gut microflora of healthy infants.Methods:Respectively collected37cases of healthy infants (21cases of vaginal delivery,16cases of caesarean birth) and from those37cases,15cases are breast-feeding infants and another22cases are bottle-feeding infants. Extracting the bacterial DNA, measuring DNA-A260, and quantify intestinal bacteria by real-time FQ-PCR.Results:The logarithms of three bacterial amounts (copies/g) in healthy infants were respectively:Bifidobacteria9.49±0.59, Lactobacilli8.58±0.32, Escherichia coli6.87±0.67;Quantitive data of the bacteria results had no differences in different mode of delivery groups and different feeding groups.Conclusions:Bifidobacteria and lactobacilli are the dominant gut microflora of the healthy infants, whereas Escherichia coli have significantly less number; Different mode of delivery and feeding had no influence on infant gut microflora. PART TWOCHANGES OF INTESTINAL FLORA IN INFANTS WITH CHOLESTATIC LIVER DISEASE AND THE CORRELATION BETWEEN INTESTINAL FLORA AND RELATED CYTOKINESObjective:To explore the changes of intestinal flora in infants with Cholestatic Liver Disease and the correlation between intestinal flora and related cytokines. To analyze the influence of probiotics in the treatment of Cholestatic Liver Disease.Methods:(1) Compare the number of three kinds of bacteria between healthy control and cholestatic liver disease using SYBR Green I Real-time FQ-PCR method. Analyze the influence of probiotics in the treatment of Cholestatic Liver Disease by comparing the number of three kinds of bacteria.(2) Detect the indexs of liver function, blood ammonia levels, and cholesterol; measure the levels of serum TGF-(31, TNF-α, and IL-6using ELISA test.Results:(1) In infants with cholestatic liver disease the number of bifidobacterium and lactobacillus significantly decreases, whereas the number of Escherichia coli significantly increases.(2) In the probiotic interference group the number of bifidobacterium and lactobacillus significantly increases, whereas the number of Escherichia coli significantly decreases.(3) Liver function decreased after therapy and the levels of blood ammonia, TNF-α, and IL-6 significantly decreased in the probiotic interference group. The correlation analysis showed that the B/E value and the IL-6levels were significantly negative.Conclusions:There is a disturbed intestinal flora in infants with Cholestatic Liver Disease and this disturbed intestinal flora can affect the related cytokines. Application of probiotics on the intestinal flora in the treatment of cholestatic liver disease may help to restore and balancing the immune function to a certain extent.