The Study Of Relationship Between Intestinal Flora And Nonalcohdic Fatty Liver Disease

Objectives To detect the amount of Escherichia coli， Lactobacillus andBifidobacteria in rabbits’ feces which on diet-induced nonalcoholic steatohepatitis andnormol using RT-PCR. Methods fourteen male New Zealand rabbits were randomlydivided into the model group and the control group, the model group was feed by high fatto form the NASH model, the control group was feed by ordinary feed, the feed lasted for12weeks, then put to execution taking a piece of liver’s tissue to make Conventionalpathology HE staining,and taking feces to extract bacteria group of DNA, according to the16S rDNA sequence to design Escherichia coli, Lactobacillus and Bifidobacterium’sspecies of specific primer, using RT-PCR for the analysis of the number of differentbacterias. Results The pathology detection of model group animals prompts hepaticsteatosis, indicating that the model success. The amounts of Escherichia coli，Lactobacillus and Bifidobacteria groups respectively （11.48±1.09，7.39±0.81）、（4.94±0.95，5.65±0.91）、（4.07±0.97，6.45±0.90）in the model group and the controlgroup，the statistical analysis shows that the amount of Escherichia coli in model group ismore than the control group’s obviously (t=3.282, P=0.013), the changing oflactobacillus and bifidobateria was not statistically significant (t1=1.204, P1=0.268; t2=0.423, P2=0.683).Conclusions the quantity of Escherichia coli obviously increases innonalcoholic steatohepatitis rabbits’ feces，it indicates hat Escherichia coli is related to theincidence of NAFLD，while Lactobacillus and Bifidobacteria groups’amount have noobvious diffierence., relation of Lactobacillus and Bifidobacteria groups NAFLD studied.