| Ganoderma lucidum, the general speices of Ganodermataceae fungi, is phylogentically affiliated to Kingdom Fungi, Basidiomycota, Basidiomycetes, Ganodermatales, Ganodermataceae. Ganoderma lucidum is one of notable medicinal fungi from of old. Due to the special ecological environment, diversity of Ganoderma lucidum species existed in Sichuan Province, China. Right now, due to lack of useful cultivation strains of Ganoderma lucidum in Sichuan province, moreover, some of the cultivated strains degenerated seriously, it is necessary to explore wild Ganoderma lucidum resource in Sichuan for further breeding. Based on this purpose,15 wild Ganoderma lucidum strains were collected from different regions in Sichuan province and used for latter genetic diversity, phylogeny and phenotype analysis. Both ITS sequencing and ISSR molecular technique were applied to reveal the genetic diversity and phylogeny of 15 wild Ganoderma lucidum strains. And mycelium growth rate, shape of fruiting body, yield of fruiting body and spores, polysaccharides and triterpenoids content were used to evaluate the agronomic traits. The main results were list as bellowing:(1)According to the ITS-PCR results, all the 15 tested strains were clustered into one group at the similarity of 0.52. At the 0.58 similarity coefficient, all the tested strains were clustered into 3 clades. Among them, strain G, X1 and X2 were fell into on group, strains G7, H2, H1, E3 and P were clustered into the second group, the rest strains were in another single group.Based on the phylogeny analysis, the results showed the 15 tested strains were phylogenetically affiliated to Ganoderma Lingzhi, Ganoderma Lucidum and Ganoderma Sinense. And among the 15 tested strains, strains like E3, G, P, L3, P1, P2, D and L1 were belong to Ganoderma Lucidum, strains including X1, G7, HI, E2, E1 and H2 were belong to Ganoderma Sinense, strain X2 was belong to Ganoderma Lingzhi.(2) The results of agronomic traits showed that mycelium growth stage, the two strains X1 and X2 were poorly performance, not covered with a seed tube; in the cultivation stage G strain mycelium inoculation 26 d covered with a bag, D last full strain bags needed 40d. Remaining strains between. After ripening culture stage, G strains appear primordia earliest, after inoculation 32d, between E2, E3 at the latest, for the 44d, the rest between the two. Fruiting bodies and spores of statistical analysis found that the largest fruiting bodies of fresh weight was from G strain, the average yield was 22.1g, the smallest was from H1 strain, with an average yield 8.9 g. The G strain had the highest biotransformation rate with an average value of 5.12%, the lowest was strain H1, with an average of 3.09%. Fruiting body weight measurement results showed that the strains P2 had the highest yield, with an average value of 1.61%, while the lowest content was in E1, which amount was 0.76%. Finally, strain G had the highest amount of triterpenoids, while the lowest was in L1 with an average amount of 2.85. While the triterpenoids content in G, G7, P2 and X1 were relatively higher than the other strains.Totally, based on the genetic diversity index, phylogeny tree, mycelium growth rate and agronomic traits, the tested strains of P2, P, G7, and E3 were selected and could be used as the parent material used in further breeding. |
PDF Full Text Request