Extraction, Separation, And Bioactivities Of Structural Polysaccharides Hydrolysate From Ganoderma Lucidum

In this study, Residue of fruiting bodies of Ganoderma lucidum after hot water extractionwas used as raw material for extracting structural polysaccharides with acid hydrolysis at high temperature and pressure, and the hydrolized Ganoderma lucidumstructure polysaccharides were isolated and purified, the separated products weresystematically studied on their anti-oxidation, immunity regulation and other aspects of biological activities.Using ganoderma lucidum residue as raw material, acidic hydrolysis of structural polysaccharides in high temperature was performed to extract functional Polysaccharides of significant free radical scavenging capacity. Based on the single factor experiments, temperature, time and acid concentration were choosen to formulate a central composite experiment design. By response analysis, the prediction models were established and the optimum process conditions were obtained as follows:temperature,131℃, Acidic hydrolysis time,4.1h, acid concentration:0.1mol/L. In these conditions, the predicted product yeild and IC50were as follows:yield:2.666%,IC50:1.039mg/ml the total free radical scavenging capacity was comparable to VC, and slightly lower than water-soluble ganoderma lucidum polysaccharide.Further research about separation and purification and identification of properties of structural polysaccharides of Ganoderma were conducted. Hydrolized Ganoderma structure polysaccharides can be separated into four components by DEAE-Sepharose Fast Flow. Two of them, GLSP1and GLSP2were then purified using Sephedex G-25column. Infrared spectroscopy analysis revealed that both components have typical absorption peaks of polysaccharide. Mn of GLSP1is1489D, and Mn of GLSP2is4913D as calculated from HPLC peak retentions. The antioxidant capacity and free radical scavenging ability of GLSP are decreased after separation while GLSP2is stronger than GLSP1.Spleen cells and macrophages were selected as two typical models to test the immune function of GLSP1and GLSP2using indice such as the appreciation rate of spleen cells, the impact of GLSP on mouse peritoneal macrophage survival activity, and NO and TNF-aproduction of PM, Both GLSP1and GLSP2could promote spleen cell proliferation.GLSP had almost no effect on the activity of peritoneal macrophages in mice, GLSP1had a stronger effect on PM than GLSP2when work directly on PM cell. but for LPS-induced PM, GLSP2cells produced more NO than GLSP1. In mouse peritoneal macrophage function experiments, GLSP2had stronger effect than GLP1, when the cells induced by LPS, GLSP2hadstronger inhibitory effect to PM cell of TNF-a than the GLSP1.