Therapeutic Effect And Its Mechanism Of Pien Tze Huang On Guinea Pig Cholecystitis

ObjectiveThe purpose of this study was to observe the therapeutic effects of traditional Chinese medicine Pien Tze Huang on animal models of cholecystitis and to elucidate its therapeutic mechanism.MethodsFor animal cholecystitis models,the guinea pigs were fed with lithocholic acid(300mg / kg body weight)for 15 days.The experimennt were divided into four groups:normal control group(n = 10),model control group(n = 20),Pien Tze Huang group(n =30),Ursodesoxycholic acid(UCDA)control group(n = 20).The guinea pig spirit,hair,and abnormal changes in feeding during the whole experiment were observed.Body weight was measured and recorded daily.After the model was finished,all groups were detected in terms of the ultrasound,pathological damage of the gallbladder,liver function [the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBil),direct bilirubin(DBil),indirect bilirubin(IBil),and total bile acid(TBA)],inflammatory factors and their autophagy related protein markers(LC3 protein)expression level.ResultsCompared with the model group,the Pien Tze Huang group had significantly better conditions with smooth hair,a good mental state,and good food consumption,and no guinea pigs died after 15 days.As was shown by ultrasound and rheography,the Pien Tze Huang group had basically normal morphology of the gallbladder and significant reductions in sagittal diameter,transverse diameter of the gallbladder and gallbladder wall thickness(p <0.05).In the terms of liver function,ALT,AST,TBiI,DBil,IBil,and TBA were significantly decreased(p<0.05).HE staining showed that Pien Tze Huang significantly improved the pathological damage of the gallbladder.The results of electron microscopy showed that the microscopic structure of the gallbladder of the Pien Tze Huanggroup significantly improved.The results of q RT-PCR showed that the expression of TNF-α and IL-1β mRNA in the Pien Tze Huang group was significantly decreased(P<0.05).Immunofluorescence histochemical examination showed that LC3 protein expression in Pien Tze Huang group was significantly increased.ConclusionThe Pien Tze Huang have protective effect on the cholecystitis model of guinea pig by improving the gallbladder morphology,the pathological injury,the micromechanism and the liver function of guinea pigs with cholecystitis.In the Pien Tze Huang group,the expression of TNF-α and IL-1β mRNA was significantly decreased(P<0.05),and the expression of autophagy-related protein marker(LC3 protein)was significantly increased.It is suggested that Pien Tze Huang has a therapeutic effect on cholecystitis,and its therapeutic mechanism is exerted by regulating pro-inflammatory factors and autophagy-related factors.