The Effect Of Qiangxin Capsule On Ca²⁺-Mg²⁺-ATP Enzyme In Myocardial Cells Of Rats With Heart Failure

Objective:To observe the effect of Qiangxin Capsule on Ca²⁺-Mg²⁺-ATPase activity in rats with heart failure,and to explore the possible mechanism of Qianxin Capsule in delaying myocardial energy metabolism during heart failure.Methods:100 male Sprague-Dawley rats were randomly selected 10 as normal group（KG）.The remaining 90 rats were treated with intraperitoneally injection with Adriamycin（ADR）to establish a model of rat with heart failure.After the successful establishment of the model,50 heart failure rats were established.The successful rats with this model were randomly divided into 5 groups: model group（MG）,trimetazidine hydrochloride group（YG）,Qiangxin capsule high-dose group（QG）,Qiangxin capsule middle-dose group（QZ）and Qiangxin capsule low-dose group（QD）.Rats were given continuous administration 4 weeks.Then the activities of Ca²⁺-Mg²⁺-ATPase,left ventricular ejection fraction,and heart weight index of myocardial cells in heart failure rats were measured.Results:（1）Comparison of myocardial mitochondrial Ca-Mg-ATPase activity: Compared with the normal group（KG）,the other groups all decreased to varying degrees.The rat of the model group（MG）,the Qiangxin capsule middle-dose group（QZ）and the Qiangxin capsule high-dose group（QG）were lower than the normal group（KG）,the difference was statistically significant（P<0.01）;The rats of the normal group（KG）,the trimetazidine hydrochloride group（YG）and the Qiangxin capsule high-dose group（QG）were higher than the model group（MG）,the difference was statistically significant（P<0.01）.（2）The effects of left ventricular ejection fraction on Echocardiographic（LVEF）: Compared with the normal group（KG）,the LVEF level of the other groups were significantly reduced.The level of the model group（MG）,the Qiangxin capsule middle-dose group（QZ）and the Qiangxin capsule low-dose group（QD）were significantly lower than the normal group（KG）,the difference was statistically significant（P<0.01）;The level of the normal group（KG）,the trimetazidine hydrochloride group（YG）,the Qiangxin capsule high-dose group（QG）and the Qiangxin capsule middle-dose group（QZ）were higher than the model group（MG）,the difference was statistically significant（P<0.01 or P<0.05）;（3）Comparison of heart weight index（HWI）:Compared with the normal group（KG）,the index of the other groups increased to some extent.The index of the model group（MG）,the Qiangxin capsule middle-dose group（QZ）and the Qiangxin capsule low-dose group（QD）were lower than the normal group（KG）,the difference was statistically significant（P<0.01 or P<0.05）;The index of the trimetazidine hydrochloride group（YG）,the Qiangxin capsule high-dose group（QG）were higher than the model group（MG）,the difference was statistically significant（P<0.05）;（4）Comparison of left ventricular mass index（LVMI）:Compared with the normal group（KG）,the index of the other groups were increased in different degree;The index of the model group（MG）and the Qiangxin capsule low-dose group（QD）were obviously higher than the blank group,the difference was statistically significant（p<0.01）;The index of the normal group（KG）,the trimetazidine hydrochloride group（YG）,the Qiangxin capsule high-dose group（QG）were higher than the model group（MG）,the difference was statistically significant（P<0.05 or P<0.01）;（5）General observation of rats: In the course of intragastric administration,rats in the normal group（KG）had good mental state and had a sensitive response;Rats in the model group（MG）had poor sperm status,unresponsiveness,and mutual biting;The rest of the rats were in a state of mental state good and eating normally.Conclusion:Qiangxin Capsule can increase the activity of Ca²⁺-Mg²⁺-ATPase in myocardial cells and improve myocardial energy metabolism.Its mechanism of action in delaying heart failure may be related to improving myocardial energy metabolism.