The Study Of Activate Spleen-energy Granules On Effect Of Gastrointestinal Hormone In Liver And Spleen Deficiency Of FD Rats

Objective:Observe the effect of Activate Spleen-energy on FD and gastrointestinal hormone.Analyze the function mechanism which expresses that Activate Spleen-energy regulates and controls the serum and the gastrointestinal hormone in tissue of FD model rats.Tentatively discuss the molecular biology mechanism which promotes Gastrointestinal Motility to provide pharmacology basis and theoretical reference for Activate Spleen-energy to prevent FD.Methods:According to the random allocation,70 SD rats are divided into normal control group(blank group)and model group.The rats of control group are 15,and the module group’s are 55.Then replicate the FD rats model with liver and spleen deficiency by adopting the compound factor based on moderate tail stimulation.After the modeling,randomly chose 5 rats from the two groups and measure the gastric emptying as well as the small intestine propulsion distance to verify whether the modeling is successful or not.Next divide the rats of the model group into several groups:there are model group,low-dose of Activate Spleen-energy group,middle-dose of Activate Spleen-energy group,high-dose of Activate Spleen-energy group and western medicine group.The number of each group is 10.And use normal saline to irrigate stomach of the rats in the blank group and model group.As for the rats in the low-dose of Activate Spleen-energy group,middle-dose of Activate Spleen-energy group and high-dose of Activate Spleen-energy group,we shall use different concentrations of Activate Spleen-particles to irrigate stomach.Afterwards,the rats from the western medicine group should be irrigated stomach with Domperidone Suspension.And the six groups are given medicine 2 times a day for 14 days.During the test,observe the general situation and pathology histology changes of the rats in every group.Moreover,the rats’ weight and appetite of each group will be recorded.After intervention,measure the gastric emptying and the small intestine propulsion distance.On the one hand,use the method of ELISA to test the content of serumMTL、GAS、CCK;on the other hand,adopt the method of RT-PCR to measure the expression level of SPmRNA and VIPmRNA in the colonic structure and gastric ant rum for all the rats.Results:(1)The Macroscopic and histopathology changes of the FD rats: contrast with the normal control group,the rats from the module group show some obvious phenomenons such as groaning when stimulating the tails,biting each other,feeling uneasy outside,showing listlessness,getting together,appearing coarse hair,the feces is dry or wet variably;while the rats in the low-dose of Activate Spleen-energy group,middle-dose of Activate Spleen-energy group,high-dose of Activate Spleen-energy group and western medicine group show gentle emotion in different degrees.Also,the activity is normal,the faeces is shaping and regular.The gastric epithelium of the blank group rats is complete according to observing the HE staining from the light microscope.What’s more,the intrinsic membrane gland arranged closely without congestion and edema;There appears the blood capillary with massive congestion in the model group rats.Besides,the epithelial cells fell off,the intrinsic membrane had internal edema and there were inflammatory cells;In a word,the gland of all the rats in every group arranged orderly,there were a few red blood cells and the epithelial cells were incomplete.(2)The changes of food-intake,weight,gastric emptying and intestinal propulsive distance of rats:compared with model group,the low-dose of Activate Spleen-energy group,middle-dose of Activate Spleen-energy group,high-dose of Activate Spleen-energy group and western medicine group rats had the rising food-intake,weight,gastric emptying rate and small intestinal propulsive rate.And the differences were statistically significant(P<0.05,P<0.01);however,take the medicine group and each dose group into compare,the differences were not statistically significant(P>0.05).(3)The content of serum MTL、GAS、CCK changes of the rats: Compared with the model group,the medicine and each dose group rat’s content of MTL and GAS rose,but the content of CCK declined,and the differences were statistically significant(P<0.05,P<0.01);and in the dose group,when compared the high-dose of Activate Spleen-energy group rats with the medicine group rats,the differences of serum content of MTL,GAS and CCK were statistically significant(P<0.05).But the differences of serum content of MTL,GAS and CCK were not statistically significant when compared the low-dose of Activate Spleen-energy group rats with the medicine group rats(P>0.05).(4)The expression level of SPmRNA and VIPmRNA in the rats’ gastric antrum and colonic tissue: compared with model group,the expression level of SPmRNA in the medicine and each dose group rats’ colonic tissue and that in the rats’ antral tissue of middle-dose of Activate Spleen-energy group and high-dose of Activate Spleen-energy group was obviously higher.And the differences were statistically significant(P<0.01,P<0.05);However,the expression level of VIPmRNA,existing in the rats’ antral tissue that from the each dose group and medicine group and the rats’ colonic tissue that from the middle-dose of Activate Spleen-energy group and high-dose of Activate Spleen-energy group was obvious lower.Also,the differences were statistically significant(P<0.01,P<0.05);Furthermore,the regulating effect for the VIPmRNA in rats’ gastric antrum and the SPmRNA in the rats’ colonic tissue of high-dose of Activate Spleen-energy group was better than that of medicine group(P<0.05),but there was no difference between low-dose of Activate Spleen-energy group and western medicine group(P>0.05).Conclusion: the Activate Spleen-energy promoted the rats’ gastric emptying and small intestinal propulsion function though the bidirectional regulation for the FD rats which were in liver and spleen deficiency.Thus,the FD rats’ food-intake and weight could be increased,the dynamic obstacles of gastrointestinal tract could be improved,and in the last,there would be new theoretical basis for the Activate Spleen-energy to prevent and cure the FD.