| Objective: This experiment used high-fat forage induction of NAF-LD rat model.Then respectively using Shiweitiaozhi decoction,Simvast-ati Bto intervene.Taking liver sample to observe histological and TG,T-C changes;Taking blood sample to detect TG,TC,ALT,AST after tre-atment.Using the technology of RT-PCR test the expressions of Matrix-metalloproteinase-2m RNA(MMP-2m RNA)and Tissue inhibitor of metaloproteinase-2m RNA(TIMP-2m RNA)in the rats liver.From this we can study the effcts of Shiweitiaozhi decoction on NAFLD rat model and it possibliable mechanism,There is hope that this study provide experimen-ttal basis for TCM prevention and treatment of NAFLD.Methods: 40 healthy male Wistar rats were fed adaptively for 1week,and then were randomly assigned into normal group,model group,Shiweitiaozhi decoction group,Simvastati group,10 rats per group.Which in addition to the normal group in ordinary feed,the remaining three groups are high fat forage.After eight weeks,Selecting one rat ra-ndomly and respectively from high-fat feed group,and Liver tissue was obtained from the rat to investigate histological changes to determine the model are successful or not.After the model was established succes-sfully,the rats in normal group and model group gavaged with Normal saline(NS),each treatment group gavaged with medicine.After 4 weeks,all rats were killed to collect liver tissue and blood specimens.Pathomo-rphological changes in liver tissue were observed by hematoxylin and e-osin(HE)staining.Triglycerides(TG),total cholesterol(TC),alanine ami-notransfe-rase(ALT),aspartate aminotransferase(AST)in the Serum were detected by Humalyzer2000 semi-automatic biochemical analyzer.TG an-d TC in the hepatic homogenate were measured by Model 722-visible S-pectrometer.The expression levels of MMP-2m RNA and TIMP-2m RNA in liver tissue were tested by reverse transcription Polymerase chain r-eaction(RT-PCR).Results:1 The result of rats liver pathology Normal group rats hepatic lobule structure is clear,the liver cells a-round the central vein arranged in the form of radiation,normal liver cells structure,no Fat droplets and inflammatory cells,nucleus was normal and located in the center of the cell,cytoplasm was abundant.In model group,fatty degeneration of hepatocytes and infiltration in inflam-matory cells,the size of liver cell increased,nucleus was pushed aside by fat vacuoles;The Liver cells of other treatment group had a trend toimprovent in different degress.2 The comparison of indexes in serum and liver tissue Compared with normal group,the rats serum TG,TC,ALT,AST and liver tissue TG,TC in model group increased significantly,The difference was statistically significant(P<0.05);Compared with model group,t-he indicators of rats serum TG,TC,ALT,AST and liver tissue TG,TC,in Shiweitiaozhi decoction and Simvastatin group are significantly lower.T-he difference was statistically significant(P<0.05);Compared with Chine-se herb medicine group,the western medicine group rats serum TG,TC decreased significantly(P<0.05);but TG,TC of the liver tissue higher tha-n Chinese herb medicine group,however the indexes ALT,AST were no difference(P>0.05).3 The expression of MMP-2m RNA and TIMP-2m RNA in liver tissue of rats The expression of MMP-2m RNA in liver tissue has no statistically significant difference.Compared with the normal group,a higher expressi-on of TIMP-2m RNA;There was statistically significant difference(P<0.05).There are differences in the ratio of MMP-2/TIMP-2(P<0.05).Com-pared with the model group,the TIMP-2mRNA expression in liver tissue of Shiweitiaozhi decoction group decreased slightly(P>0.05).The exprees-ion in the ratio of MMP-2/TIMP-2 of Shiweitiaozhi decoction group higher than the model group(P<0.05).Compared with the model group,the TIMP-2m RNA expression in liver tissue of Simvastatin group increased o-bviously(P<0.05).The expreesion in the ratio of MMP-2/TIMP-2 of Si-mvastatin group were no difference(P>0.05).The expression of TIMP-2m RNA in western medicine group was higher than Shiweitiaozhi decoctio-n group(p<0.05),but the ratio of MMP-2/TIMP-2 lower than it(P<0.05).Conclusion:1 The serum TG,TC,AST,ALT and hepatic TG,TC in NAFLD rat model significantly increased relative to normal group,which maybe the reason that hepatocyte fatty,inflammation and fiber.2 One of the possible mechanisms is the expression of MMP-2 in model group decreased and TIMP-2 has a tendency to rise has caused hepatocyte fatty,inflammation and fiber.3 Both Simvastatin and Shiweitiaozhi decoction could reduce the T-G and TC levels in NAFLD rats,B decreased the TG,TC in liver tissue was more obvious than simvastatin.4 Shiweitiaozhi decoction may be rectify metabolic disorder of lipid and decrease the deposit of lipid in the liver,Slow down the disease processes of the fibrosis by increasing the expression of The ratio of M-MP-2/TIMP-2,but the further research are needed.Meanwhile,this study provides the experimental evidence for Chinese medicine treatment o-f NAFLD. |
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