The Physicochemical And Biological Characteristics Of Coaxial Electrospun Barrier Membrane Loaded BMP-2 And IGF-1

The fiber membrane prepared by coaxial electrosp un technology has the ability of simulating with the extracellular matrix and releasing drugs.Bone morphogenetic protein-2(BMP-2)and insulin like growth factor-1(IGF-1)also have good ability of bone induction.This study is to fabricate three-dimensional nanofiber membranes loaded with BMP-2 and IGF-1 by coaxial electrospun technology and assess its physicochemical and biological properties in vitro.Firstly four experiment group of membranes loaded BMP-2/IGF-1 were fabricated successfully.Apparent characteristics of membranes were assessed by scanning electron microscope(SEM),transmission electron microscopy(TEM)and laser confocal microscope(LSCM).Then the physicochemical and drug release properties were evaluated respectively by contact angle(CA),mechanical property test,X-ray diffraction(XRD)analysis and ELISA K its.Moreover,bone marrow derived mesenchymal stem cells(BMMSCs)seeded onto the membranes were used to estimate its biological properties by cell viability and alkaline phosphatase(ALP)activity.The experiment results showed that four groups of membranes loaded dual-factors with different flow ratio(shell/core)presented uniform diameters and core-shell structures.With the acceleration of shell solution flow rate,the contact a ngle and mechanical properties of fibrous membrane were increased,and the addition of dual-factor had no effect on the fiber structure.Each drug loaded membrane showed a gradually increasing release curve with varying degrees of burst release and sustained release,among which the drug loading capacity and sustained release performance of membranes were better than that of other groups with the core-shell flow ratio of 1:10.BMMSCs viability and ALP activity on fibrous membrane with flow ratio of 1:10 wit hin 14 days were also higher than that of other experiment groups.At the same time,the fiber membrane of F3 group could effectively prevent the perforation of periodontal ligament fibroblasts.Therefore,the optimal parameters were chosen ultimately acco rding to the proper physical and chemical characteristics and biological properties of the membrane.The results imply that the membrane loaded BMP-2/IGF-1/BSA with optimum parameter not only meet the requirements of barrier membrane for guided bone regeneration(GBR)but also release multiple factors for osteoconduction and osteoinduction.