Study On The Construction Of Drug Loaded Fiber And Its Property Of Carrying Ginsenoside Rg-1 By Coaxial Electrospinning

Objective: Preparation of carrying ginsenoside Rg-1 of SF/PLCL composite drug loaded fiber scaffold using coaxial electrospinning, comparing the different core shell rate ratio fiber in surface morphology, hydrophilicity, thermal and mechanical stability and crystal structure impact performance, to provide preliminary data for tissue engineering in the field of drug delivery.Methods: Firstly at the concentration of 8%, quality ratio of 30:70 SF/PLCL mixed solution as the shell spinning materials, 10 – 4mol/L ginsenoside Rg-1 as the core layer of the spinning material, shell advancing velocity is 1.0 m L/h, and core layer speed are 0.167 m L/h, 0.125 m L/h, 0.1m L/h, 0.083 m L/h, respectively. Composite carrier fiber scaffolds with different core shell rate ratios were prepared by coaxial electrospinning technology. Scanning electron microscope and transmission electron microscope were used to observe the surface morphology and internal structure of the fibers; crystal structure characteristics of X- ray diffraction test the sample; integrated thermal analyzer test sample thermal stability; hydrophilic property of the samples were measured using contact angle measuring instrument; mechanical properties of the samples were measured by universal mechanical testing machine; UV spectrophotometer was used to measure Chinese medicine monomer ginsenoside Rg-1 drug content in the composite fibers and the cumulative release rate.Results: 1) The Scanning electron microscopy and transmission electron microscopy showed that the surface of the composite fiber was smooth and uniform in diameter, and had a three-dimensional network structure. There are obvious core-shell structure, only a small part of the fiber core layer deviation, which means the core layer of drug coated uneven, left and right sides of the shell thickness is inconsisten. And with decrease of core shell rate ratio, the diameter of the composite fibers is increased. 2) X-ray diffraction show silk fibroin transformed into a stable β-sheet conformation, enhance the degree of crystalline materials, fibrous membrane of different core-shell rate ratio appeared a new diffraction peaks at around 16.7 °, but the pattern curve generally is similar, only the diffraction peak vary on width and height, the other obvious diffraction peak does not appear, material propertie is stable. 3) The contact angle showed that the composite fiber membrane with different core shell rate ratio was 124.83°±2.87,126.34°±2.29,128.54°±4.06 and 129.65°±1.60, respectively. Each group of materials hydrophilic angle values are larger than 90°, the material is hydrophobic, with increasing of core shell rate ratio, the material of hydrophilicity increases. 4) Ginsenoside Rg-1 powder of mass loss at the temperature range from 2℃ to 148℃, the weight loss of the fiber membrane at a temperature range from 300℃ to 420℃, materials has good thermal stability. 5) Mechanical properties show that the elongation at break and elastic modulus increases and the tensile strength decreases with the decrease of the ratio of core shell rate. 6) Under the same conditions of drug release, F4 fiber membrane loading too little, F2 and F3 drug release is relatively stable, although in the early release also has burst phenomenon, but in the latter part of the release process, F3 can be effectively used to release the drug is better than F2. As the ratio of the core shell rate decreases, the drug release rate is accelerated.Conclusion: It is successfully prepared to load ginsenoside Rg-1 of SF/PLCL composite fiber scaffold with good crystallinity, excellent thermal stability, good hydrophilic and mechanical properties. The drug can be coated in the fiber core layer, forming a reservoir type drug release system, which has potential application value and development prospects in the field of drug release.