Expression And Correlation Of Folate Receptor α And CA125 In Endometrial Carcinoma

BackgroundEndometrial carcinoma is a kind of epithelial malignant tumor which originated from the endomtrium,the incidence of it has been the first of female gentital tract malignant tumors in some European developed countries.In recent years,the incidence rate is on the rise in the worldwide,and become more younger.The etiology and pathogenesis is not completely clear,there is no breakthrough in the the treatment progress,and the postoperative survival rate remains to be improved.At present,the diagnosis of endometrial carcinoma mainly depends on symptoms,ultrasound,diagnostic curettage,tumor markers and other auxiliary examinations.Diagnostic curettage is the most common and valuable diagnostic method.To improve the quality of life and the survival of patients with endomtrial carcinoma,early detection,early diagnosis and early intervention is the key.Recently,it has been reported that folate receptor alpha(FRa/FRA)is highly expressed in most endometrial carcinoma tissues,and is expected to be the new tumor marker.Folate receptor alpha(FRa/FRA)is a glycosylphosphatidylinositol-anchored glycoprotein,it shows restricted expression on the apical surface in normal tissues,but to be overexpression in epthelial malignant cells.Because of the expression variation and its specificity in malignant epithelial tissues compared with noraml tissues,it’s expected to be the new biological markers in assisting tumor diagnosis,monitoring and prognosis.Our team’s early study found that serum FRA in patients with endomatrial carcinoma was significantly expressed,then,what condition of FRA expression in tissues of endometrial carcinoma is?CA125,the most common gynecological tumor markers,has been widely applied to tumor diagnosis and tracking in urogenital system and digestive system.At present,the expression of CA125 in endometrial carcinoma is mostly confined to the serology.However,most scholars considered that serum CA125 concentration can not respond to the degree of tumor progression accuratelly.Thus,what is the significance of CA125 expression in endometrial lesions tissues?Does it have a correlation with FRA expession in endometrial carcinoma tissues.Whether it could provide a valuable predication for the diagnosis and prognosis?Objective:Detecting the expression of FRA and CA125 in different endometrial lesions by immunohistochemical assay and observing its relationship with the clinicopathological characteristics,so that to explore the clinical significance and potential mechanism in the progress of endometrial carcinogenesis.Method:A tolal of 60 cases of endometrial carcinoma were collected and 46 cases of endometrial hyperplasia and 10 cases of normal endometrium were randomly selected.All specimens were fixed in paraffin-edmbedded tissues.And the expression of FRA and CA125 in tissues was detected by immunohistochemistry.Positive staining for the cell membrane showed brown and browm particles occured in cytoplasm,no staining represented negative expression.Results:1 The positive expression rates of FRA in normal endometrial tissues,endometrial hyperplasia tissues and endometrial carcinoma tissues were 10.0%,45.7%and 93.3%,respectively,and the FRA was strongly expressed in endometrial carcinoma(high expression rate for 65.0%),which was higher than that of hyperplasia and normal group(P<0.05).2 The positive rate of FRA in complex endometrial hyperplasia tissues was higher than that in simple type,and the expression rate of atypical hyperplasia was significantly higher than that in dysplasia(P<0.05).In the endometrial carcinoma group,the statistically significant relationship to age,FIGO stage and differentiation degree was observed(P<0.05).3 CA125 in the nomal endometrium is low expression,while it’s high expression in endometrial hyperplasia tissues and endometrial carcinoma tissues.There was a weak positive correlation between FRA and CA125 in the process of endometrial carcinogenesis.(r=0.204,P=0.028)Conclusion:1 The expression of FRA in endometrial tissue was gradually up-regulate from the normal proliferative to hyperplasia,atypical hyperplasia then carcinoma,which suggested that FRA might participate in the endometrial lesions and even carcinogenesis.So as to provide a theoretical basis for targeted diagnosis of endmetrical carcinoma.2 The expression of FRA in endometrial carcinoma was significantly higher than that in non-cancerous tissue,and it was correlated with the differentiation and staging,which suggested that FRA might be a new therapeutic target for endometrial carcinoma.3 FRA and CA125 showed an upward trend in the process of endometrial carcinogenesis,and the combination of FRA and CA125 was superior to single index in the diagnosisi of endometrial carcinoma.