Expression Of MIF And NF-κB In Endometrial Hyperplasia And Endometrial Carcinoma Ⅰ And Their Correlation

Objectives:By detecting the expression of MIF and nuclear factor-KappaB (NF-κB) in endometrial hyperplasia and endometrial cancer I to analyze their relationship with clinical-pathologic parameters and their expressed relationship in endometrial hyperplasia and endometrial cancer I, thereby to investigate the role of MIF and NF-κB in the endometrial disease development.Methods:Immunohistochemistry was utilized to measure levels of expression of MIF and NF-κB/P65in the endometrial tissue specimens. The endometrial tissue specimens were obtained from22cases endometrium with proliferative phase,25cases with simple hyperplasia and complex hyperplasia,15cases with atypical hyperplasia,65cases with endometrial carcinoma I.Results:Expression of MIF can be seen in all of the endometrium tissues including normal endometrium, endometrial hyperplasia and endometrial carcinoma. The positive expression of MIF in normal endometrium was40.90%, the positive expression of MIF in endometrial hyperplasia was57.45%, and the positive expression of MIF in endometrial carcinoma I was87.69%. The positive expression of MIF in the three teams has significant statistic difference among them (P=0.000, P<0.01). The percentage of MIF positivity in simple hyperplasia and complex hyperplasia teams was lower than atypical hyperplasia team, and there was statistic difference among them (46.67%,76.47%, P=0.047, P<0.05). MIF expression did not correlate with any other clinical pathologic parameters such as histological grade, FIGO stage, myometrial invasion or lymph node status (P>0.05).The expression of NF-κB/P65was seen in normal endometrium,tissues of endometrial hyperplasia and tissues of endometrial carcinoma I. The positive expression of NF-κB/P65in normal endometrium was31.82%, the positive expression of NF-κB/P65in endometrial hyperplasia was38.30%, and the positive expression of NF-κB/P65in endometrial carcinoma was72.31%. The positive expression of NF-κB/P65in the three teams had significant statistic difference among them (P=0.000,P<0.01). The percentage of NF-κB/P65positivity in simple hyperplasia and complex hyperplasia teams was lower than atypical hyperplasia team, and they also had statistic difference between them (26.67%,58.82%, P=0.029, P<0.05). The percentage of NF-κB/P65positivity in moderately and poorly differentiated tissues was higher than that in well differentiated endometrial carcinoma tissues, and they had statistic difference (86.67%,60.00%, P=0.017, P>0.05). NF-κB/P65expression did not correlate with any other clinical pathologic parameters such as FIGO stage, myometrial invasion or lymph node status (P>0.05).There was a positive correlation between MIF and NF-κB/P65expression in endometrial hyperplasia tissues (r=0.324, P=0.026, P<0.05) and endometrial carcinoma I tissues (r=0.291, P=0.019, P<0.05) by the analytical method of Spearman.Conclusion:1. In normal endometrium, tissues of endometrial hyperplasia and tissues of endometrial carcinoma, the expression level of MIF showed an increasing. In the endometrial hyperplasia, the positive expression of MIF in atypical hyperplasia was higher than the group of simple hyperplasia and complex hyperplasia. But in the endometrial cancer, MIF is not correlated with histological grade, FIGO stage, myometiral invasion, or lymph node status. So we concluded that MIF may be the early event of the endometrial disease development to endometrial cancer I.2. In normal endometrium, tissues of endometrial hyperplasia and tissues of endometrial carcinoma, the expression level of NF-κB/P65also showed an increasing. In the endometrial hyperplasia, the positive expression of NF-κB/P65in atypical hyperplasia were higher than the group of simple hyperplasia and complex hyperplasia. NF-κB/P65expression was significantly higher in moderately and poorly differentiated endometrial carcinoma tissues than that in well differentiated tissues, which suggested up-regulation of NF-KB/P65may play an important role in the tumorigenesis and development of endometrial carcinoma I.3. Both MIF and NF-κB/P65showed an abnormal increasing tendency in endometrial hyperplasia and endometrial carcinoma I.NF-κB/P65is from well-differentiated to moderately and poorly differentiated endometrial carcinoma Ⅰ, there was a positive correlation between MIF and NF-κB/P65expression in endometrial disease development. However, further study should be performed to confirm this conclusion and elucidate the precise role of MIF and NF-κB/P65in the initiation and promotion of endometrial disease development.