Clinical Observation Of Liangxue Siwu Decoction On Treating Blood-heat Psoriasis Vulgaris And The Effect On CD4~+, CD8~+ T Lymphocyte In Peripheral Blood

1 Objective To observe the clinical effect of Liangxue Siwu Decoction in the treatment of blood-heat psoriasis vulgaris and to observe CD4~+,CD8~+ T lymphocytes and CD4~+/CD8~+ ratio changes in peripheral blood before and after treatment,in order to explore the partial mechanism of treatment of Liangxue Siwu Decoction for blood-heat psoriasis vulgaris.2 Methods60 patients into the final research with blood-heat type of psoriasis vulgaris were randomly divided into treatment group 31 cases and control group 29 cases.There were20 cases from normal examination in the normal control group.The treatment group was given Liangxue Siwu Decoction with addition and subtraction according to the syndromes,and the control group was given hospital preparation of compound Zeqi granules to treat.The two groups were treated for 8 weeks.Before and after treatment,the patients’ PASI score and TCM syndrome score were recorded.The PASI score,TCM symptom score between the two groups and within the two groups and clinical efficacy of the two groups were statistically analyzed.Two groups treated for 1 months and two months must examine liver function,blood routine,urine routine,stool routine.The treatment group and control group were extracted 2ml venous blood before and after treatment.20 cases of normal people were also extracted 2ml venous blood from elbow.By the technology of flow cytometry,CD4~+,CD8~+ T lymphocytes in peripheral blood and CD4~+/CD8~+ ratio were determined.Statistically analyze differences between the treatment group and the control group and within the two groups before and after treatment in each experimental index.Analyze and compare the differences between the treatment group before and after treatment and the normal control group and between the normal control group and the control group before and after treatment in each experimental index.3 Results3.1 PASI score and TCM symptom score of the treatment group and the control group before and after treatment were statistically significant(p<0.05).Between the treatment group and the control group before and after treatment,there was no significant difference in PASI score and TCM syndrome score,(p>0.05).3.2 Patients in the treatment group 31 cases of blood-heat psoriasis vulgaris patients,of which 5 cases were cured,14 cases markedly effective,7 cases effective,5 cases ineffective,the total efficiency is 83.87%;the control group of 29 cases of blood-heat psoriasis vulgaris patients,of which 4 cases were cured,11 cases markedly effective,8cases effective,6 cases ineffective,the total efficiency is 79.31%.There was no significant difference in clinical efficacy between the two groups(p>0.05).3.3 Before treatment,the CD4~+ content and CD4~+/ CD8~+ ratio in the treatment group and the control group were lower than those in the normal group(p<0.05),and the CD8~+content in the treatment group and the control group was higher than that in the normal group(p<0.05).After treatment,CD4~+ content and CD4~+/ CD8~+ ratio of treatment group was higher than before(p<0.05),but still lower than the normal level(p<0.05).CD8~+content was lower than before(p<0.05),but still higher than the normal level(p<0.05).CD4~+ content after treatment in the control group had no obvious change compared to that before treatment(p>0.05).CD8~+ decreased(p<0.05),but still higher than the normal level(p<0.05).CD4~+/ CD8~+ ratio increased(p<0.05),but was still lower than the normal level(p<0.05).The three indicators of the treatment group and the control group after treatment were not statistically significant(p>0.05).3.4 Two groups are of good safety,no abnormal liver and kidney function,abnormal blood routine,abnormal urine routine,and abnormal stool routine.4 Conclusion Liangxue Siwu Decoction in the treatment of blood-heat psoriasis vulgaris is effective and safe.The pathogenesis of blood-heat psoriasis is probably related to the abnormal immune response of CD4~+ and CD8~+T lymphocytes.Liangxue Siwu Decoction in the effective treatment of blood-heat psoriasis vulgaris may play a role by regulating CD4~+and CD8~+T cells in peripheral blood.