Effect Of FBXO11 Gene On Migration Of Lung Adenocarcinoma A549 Cells

Objective To explore the effects of the ubiquitin ligase complex of SCF(Skp1–Cul1–F-box-protein specificity)subunit FBXO11 on epithelial mesenchymal transition and lung adenocarcinoma A549 cells migration.Methods The A549 cell line was cultured and divided into blank group,negative control RNA group and si RNA-Fbxo11 group.Negative control RNA and si RNA-Fbxo11 were transfecected into A549 cells by Lipofectamine 2 000 respectively.Using Trizol reagent cells extracted total RNA,levels of m RNA of Fbxo11 was measured by RT-PCR and the expression of Fbxo11 and Snail was detected by Western blot in the transfected cells.The migration of A549 cells was evaluated by wound healing assay.Results(1)Compared with blank group and negative control RNA group,the levels of m RNA and the expression of Fbxo11 in si RNA-Fbxo11 group was remarkably down-regulated(P<0.05),and compared with blank group and negative control RNA group,there was no statistically significant difference.Compared with blank group and negative control RNA group,the expression of Snail in si RNA-Fbxo11 group was remarkably increased(P<0.05),and compared with blank group and negative control RNA group,there was no statistically significant difference.Compared with blank group and negative control RNA group,the migration distance in si RNA-Fbxo11 group was remarkably increased(P<0.05),and compared with blank group and negative control RNA group,there was no statistically significant difference.Conclusion Fbxo11 might inhibit the migration of lung adenocarcinoma A549 cells via the regulation of Snail.