Hsa-let-7e-5p Inhibits The Migration Of Lung Adenocarcinoma Cells By Targeting Deltex2

At present,cancer has become one of the main causes of human death in the world.Lung cancer is one of the malignant tumors with the highest incidence and mortality in the world,and the incidence is increasing year by year.According to different pathological types,lung cancer can be divided into small cell lung cancer and non-small cell lung cancer.Among them,non-small cell lung cancer accounts for more than 80%,and lung adenocarcinoma is the most common type of non-small cell lung cancer.Although the treatment of lung cancer is constantly improving,it has not fundamentally changed the high incidence and mortality of lung cancer.The characteristics of lung cancer such as easy metastasis and drug resistance are important reasons for tumor recurrence.Therefore,the mechanism of the occurrence,development,and metastasis of lung cancer still needs to be further studied.miRNA is a class of non-coding single-stranded RNA molecules with a length of about 22 nucleotides encoded by endogenous genes.There have been a large number of literature reports that it is closely related to tumor cell proliferation,differentiation,apoptosis,migration,invasion and cell cycles.In this paper,by analyzing the miRNA chip data of lung adenocarcinoma and normal controls,we screened 576 miRNAs that are differentially expressed in lung adenocarcinoma.After further verification by QRT-PCR and literature research,we selected the low-expressing hsa-let-7e-5p in lung adenocarcinoma for research.We further analyzed the expression of hsa-let-7e-5p in lung adenocarcinoma tissues in the TCGA database,and found that hsa-let-7e-5p is indeed at a low expression level in lung adenocarcinoma tissues,and has statistical significance.We tested the endogenous expression of hsa-let-7e-5p in lung adenocarcinoma cells and found that hsa-let-7e-5p is down-regulated in lung adenocarcinoma cells.We found that overexpression of hsa-let-7e-5p can inhibit the migration of lung adenocarcinoma cells by the wound healing assay.We predicted the possible target genes of hsa-let-7e-5p through three miRNA target gene prediction websites and verified the intersection of them.Combined with literature research,we further analyzed the possible target gene DTX2 through western blot and dual-luciferase reporter assays and verified that DTX2 is the target gene of hsa-let-7e-5p.We analyzed the expression level of DTX2 in lung adenocarcinoma tissues in the TCGA database,and found that DTX2 was expressed at a high level in lung adenocarcinoma,and it was statistically significant.We tested the endogenous expression level of DTX2 in lung adenocarcinoma cells and found that DTX2 is highly expressed in lung adenocarcinoma cells and can promote the migration of lung adenocarcinoma cells.Therefore,we found that hsa-let-7e-5p is down-regulated in lung adenocarcinoma,and it is possible to inhibit the migration of lung adenocarcinoma cells by directly targeting DTX2.This study may play very important role on subsequent research about the functions and mechanisms of hsa-let-7e-5p and DTX2 in lung cancer.