Research On The Animal Models Of Kidney Deficiency And Phlegm Dampness Pattern Of The Obese PCOS

[Objective]:To screen out the best animal models which are combined with the Kidney Deficiency and Phlegm Dampness (KDPD) of TCM and the clinical and metabolic characteristics of PCOS. And explore the pathogenesis of obese PCOS by studying the expression level of Bax, Bcl-2, GDF-9 and BMPR-2 in the ovary of PCOS rats.[Method]:It chose 42 and 23 days old female rats each 70 to take vaginal smear examination for 5 days, and selected these who have normal estrum. The 42 days old rats were randomly divided into Blank Control Group, high fat model group (MG), Letrozole MG, and etc; and the 23 days old rats were randomly divided into BCG, high fat MG, DHEA MG, and etc. Respectively adding high fat emulsion based on the Letrozole, DHEA for 21 days, then observed the general condition and estrum of rats, and measured the Weight Difference (WD), Organ Index (01) and the level of insulin (fins,2hins, FPG and etc.), serum sex hormone levels (E2, T, LH and etc.) and lipid levels (TC, TG, HDL and etc.), and inspect the ovaries. The ovarian tissues were observed under the microscope after hematoxylin-eosin (HE) staining, and the expression levels of Bax, Bcl-2, GDF-9 and BMPR-2 in the ovary of PCOS rats were detected by immunohistochemistry method.[Result]:(1) Effects on estrum:The estrum of Letrozole MG, Letrozole model Obesity Group(OMG), DHEA MG and DHEA OMG are in disorder, extends or under oestrus. The ovulation rate of rats was significantly decreased, the difference was statistically significant (P<0.01).(2) Effect on weight:The weight of Letrozole MG, Letrozole OMG, DHEA MG and DHEA OMG are significantly increased after treatment, and the WD also, the difference was statistically significant (P<0.01).(3) Effect on Organ Index (01):The uterine OI decreased significantly in Letrozole MG and Letrozole OMG, and the ovarian OI increased significantly, the difference was statistically significant (P<0.01). The uterine OI increased significantly in DHEA MG and DHEA OMG, the difference was statistically significant (P<0.05).(4) Effects on blood glucose and insulin:The FINS and FPG increased in Letrozole MG and Letrozole OMG, the difference was statistically significant (P<0.05), and 2hfins increased significantly, the difference was statistically significant (P<0.01). the FINS and HOMA index increased in DHEA MG and DHEA OMG, the difference was statistically significant (P<0.01).(5) Effects on sex hormones:The E2 and LH/FSH increased in Letrozole MG and Letrozole OMG, the difference was statistically significant (P<0.05), T and LH also rose, and the difference was statistically significant (P<0.01). The E2, T, LH and etc. increased significantly in DHEA MG and DHEA OMG, the difference was statistically significant (P<0.01).(6) Effect on blood lipid:The TC and TG increased in Letrozole MG and Letrozole OMG, meanwhile HDL decreased, the difference was statistically significant (P<0.05). TC and TG increased in DHEA MG and DHEA OMG, the difference was statistically significant (P<0.05).(7):Effect on ovarian morphology:In Letrozole MG and Letrozole OMG, the number of primary follicles, secondary follicles, mature follicles and corpus lutein decreased, meanwhile the number of polycystic follicular increased, the difference was statistically significant(P<0.05), and the number of corpora atretica increased significantly, the difference was statistically significant(P<0.01). In DHEA MG and DHEA OMG, the number of mature follicles and corpus lutein decreased, the difference was statistically significant(P<0.05), and the number of corpora atretica increased significantly, the difference was statistically significant(P< 0.01).(8) The expression of Bax and Bcl-2:The expression of Bax presented high level, meanwhile the expression of Bcl-2 was lower in Letrozole MG, Letrozole OMG, DHEA MG and DHEA OMG. the difference was not statistically significant(P> 0.05).(9) The expression of GDF-9 and BMPR-2:The expression of GDF-9 and BMPR-2 presented low level in Letrozole MG, Letrozole OMG, DHEA MG and DHEA OMG, the difference was not statistically significant(P>0.05).[Conclusion]:(1) Letrozole improved method and DHEA improved method are both in line with the KDPD of TCM combination with PCOS animal model.(2) Letrozole improved method is more emphasis on the blood lipid, insulin resistance, ovarian morphological changes in obese PCOS rats. (3) DHEA improved method was more emphasis on serum sex hormone levels and ovarian morphological changes in PCOS rats.(4) It can be speculated that the expression of Bax presented high level in the obese with PCOS animal model, and Bcl-2, GDF-9, BMPR-2 all showed a low level of expression.