Expression Of Has-miR-3646 In Human Breast Cancer And Its Effect On Cell Proliferation, Migration And Invasion Of Human Breast Cancer

Objective To investigate the expression of Hsa-miR-3646 in human breast cancer tissues and adjacent tissues and in breast cancer cells and normal breast epithelial cells. To study the influence on proliferation, migration and invasion of breast cancer cell lines, in order to clarify to provide a theoretical basis in the role in the development and regulation of breast cancer. Methods 1. QRT-PCR was used to detect Hsa-miR-3646 in 30 cases of human primary breast cancer tissue and the corresponding adjacent normal tissue and its expression in human breast cancer cells MCF-7 and MDA-MB-231 and MCF10A in normal breast epithelial cells.2.Mimic miR-3646 and inhibitor miR-3646 were transfected into breast cancer cells MCF7 and miR-3646 by transient transfection, and the expression of and MDA-MB-231 were over expressed or silenced.3 The effect of miR-3646 on the proliferation of breast cancer cells was detected by in vitro cell plate clone formation assay and MTT assay;4.The effect of miR-3646 on invasion assay invasion of breast cancer cells.5.The effect of miR-3646 on the migration ability of breast cancer cells was detected by cell scratch test. Results 1.The expression of Hsa-miR-3646 in human breast cancer tissue was higher than that in adjacent normal breast tissue (p<0.01), and the expression of MDA-MB-231 in and MCF cells was higher than that in MCF10A (p<0.01).2. Compared with the control group (transfected with Mir NC), proliferation ability of breast cancer cells transfected with miR-3646 mimic increased significantly (P< 0.01), and transfected miR-3646 inhibitor group breast cancer cell proliferation decreased significantly (P<0.01).3. Transwell invasion assay showed that breast cancer cells transfected with miR-3646 mimic across cell membranes are significantly higher than in control group (P< 0.01), and the number of cell invasion transfection miR-3646 inhibitor group breast cancer cells was significantly less than that of the control group (P< 0.01).4.Cell scratch wound experiment, migration distance of breast cancer cells transfected with miR-3646 mimic transfected miR-3646 NC group (P<0.01) were significantly higher than that in, and transfection miR-3646 inhibitor group breast cancer cell migration distance was significantly lower than that of the transfected miR-3646 NC group (P<0.01).Conclusion The expression of Hsa-miR-3646 is upregulated in human breast cancer tissues and cancer cells; Hsa-miR-3646 promotes proliferation, migration and invasion ability of MDA-MB-231 and MCF7 in breast cancer cells, it play a important role in the development of breast cancer.