Studies On Immunomodulatory Activities Of Fungus Polysaccharides

Fungus polysaccharides are known as the "biological response regulator". As the immunopotentiator and immune activator, fungus polysaccharides have many biological activities, and one of the significant biological activities is immunoregulation. But a single fungus polysaccharide usually only has obvious function to a certain physiological effect.In recent years, the researches on fungus polysaccharides tend to immunological effect of polysaccharide mixtures according to the specific proportion. The effect of the polysaccharide mixture with the collaborative pharmacological activities may be better than single polysaccharide.The purpose of this study is to evaluate the immunoregulatory effect of several kinds of fungus polysaccharides, and compared with the polysaccharide mixture. The regulation of fungus polysaccharides to immune function were investigated through a series of cell experiments and animal experiments. The achievements of this thesis mainly included:1) Studies on effects of fungus polysaccharides on mouse immune cells: Fungus polysaccharides at high concentration could inhibit the proliferation of RAW264.7cells, whereas, stimulatory effects happened at low concentration. There were almost no significant influences on the proliferation of mouse splenic lymphocytes treated with fungus polysaccharides at 10~200 μg/m L. But fungus polysaccharides could promote the mature and activation of lymphocytes due to increasing the proportion of T cells and macrophages in the splenic lymphocytes.2) Studies on effects of fungus polysaccharides on immunosuppressed mice: The mice treated with fungus polysaccharides could recover body weight, index of immune organs, phagocytosis of macrophages, the level of serum immunoglobulins and cytokines in immunosuppressed mice.3) Studies on effects of fungus polysaccharides on influenza vaccine against influenza virus: Fungus polysaccharides could synergize the effect of inactive influenza vaccine, which could improve the survival rate of infected mice, and decrease pulmonary infection. The polysaccharides combined with traditional vaccine had elicited stronger specific antibody response in infected mice. The polysaccharide mixture had the synergistic effects against influenza virus.In summary, lentinan, tremellan and pachymaran present immunomodulatory activities,whereas a better immunomodulatory capability was exhibited in the polysaccharide mixture group.