Preliminary Screening Of Differentially LncRNA-mRNA Coexpression Microarray In Hirschsprung’s Disease

Objective:To assay differentially expressed lnc RNA and m RNA between HD colon specimens proximal anastomosis andstenotic segment.,explore the role and its mechanism of the lnc RNA in HD by bioinformatics knowledge, provide experimental basis for the early diagnosis and early treatment for HD.Methods: 17 cases from October 2013 to September 2015 diagnosised as HD in the Affiliated Hospital of Zunyi Medical College were collected. Five cases（a total of 10 cases of bowel） were sent for human lnc RNA-m RNA expression microarray screening, Agilent genespring software was used to analysisdifferentially expressed lnc RNAs and m RNA. the chip resultwas validatedby quantitative real time-polymerase chain reaction.Bioinformatics was used to analyzethe role of these lnc RNAplayin HD.Results: lncRNA expression chip display that in HD stenotic segment 353 lnc RNA were significantly up-regulated expression, 474 were down-regulated（selection criteria by Fold change> 2, P < 0.05）. There were 865 significantly up-regulated expression m RNA,and 461 were down-regulated expression（selection criteria by Fold change > 2, P < 0.05）. q RT-PCR were used to validate the expression of 6 lnc RNAs, including TCONS₀0029197 TCONS₀0029197, ENST00000445908.1, ENST00000455286.1.The results show TCONS₀0029197 ? ENST00000445908.1 ? ENST00000455286.1 were significantly up-regμlated expression and ENST00000579378.1 was significantly down regulated, P < 0.05,so we think the screening results are coincide. Although the expression of ENST00000438136.1 in Hirschsprung’s disease was up-regulated, but its expression difference was not significant, P>0.05, the difference was not statistically significant. We failure of the TCONS₀0028253 primers disign. so didn’t analyze it.Conclusion:There are many differential expressed lnc RNA and m RNA between HD colon specimens proximal anastomosis and stenotic segment. m RNA and lnc RNA have a wide range of links, may be through the regulation of cell proliferation, differentiation and other effects of the corresponding m RNA to participate in the pathogenesis of HD.