| Objective: To study the expression level of intercellular cell adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1) and tumor necrosis factor-α(TNF-α) in unexplained recurrent spontaneous abortion(URSA) patients and to lay the foundation for the analysis of it role in the pathogenesis of unexplained recurrent spontaneous abortion.Methods: 30 cases of URSA patients and 30 cases of normal early pregnancy abortion patients were selected as experimental group and control group, respectively. Expression of ICAM-1, VCAM-1 and TNF-α in villus and decidua tissue of patients were detected by immunohistochemical method. Concentration of ICAM-1, VCAM-1 and TNF-α in serum of patients were determined using enzyme-linked immunosorbent assay(ELISA).Real-time polymerase chain reaction(Real-time PCR) was used to analysis the expression level of ICAM-1 gene in villus and decidua tissue of patients.Results: Compared with the normal early pregnancy abortion group, the expression of ICAM-1 and VCAM-1 in serum decreased significantly(p<0.01) with(74.0 ± 16.2) vs.(100.8 ± 14.4) 、(837.3 ±189.1) vs.(1140.2 ±160.7),while the expression of TNF-αincreased significantly(p<0.01) with(8.9 ± 2.1) vs.(5.4 ± 1.4); The results of immunohistochemistry studies shown similar expression trend of the three facters in the villus tissue of aborted embryos as in the serum, and all the above data were of significant difference with P<0.01. The results of Real-time PCR indicated the decreased gene expression of ICAM-1 in the experimental group(p<0.05).Conclusion: There were abnormal expression of ICAM-1, VCAM-1 and TNF-αin URSA patients, which suggesting that it may be closely related to the occurrence of unexplained recurrent spontaneous abortion The abnormal expression of ICAM-1, VCAM-1 and TNF-αcould be used as the detection indexes of early abnormal embryonic development and may provide references for the following blocking or immune regulation treatment. |
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