| Object:In this study, we replicated the VX-2 renal cell carcinoma model in rabbits, to study the dynamic evolution of different sizes of polymer nano micelles in the experimental animal tumor model, and to lay the foundation for the study of the human tumor samples. Methods:1.30 rabbits with the same body weight(2.2kg) were selected; improved open surgery was implanted in the left kidney with the VX-2 tumor mass. Then the rabbit model of renal cell carcinoma was established. Ultrasound monitoring of tumor growth and tumor characteristics, the law of tumor growth was studied and the scientific modeling methods will be summarized.2. A total of 18 rabbits were selected with the appropriate tumor, divided into 3 groups according to the different drugs. Infusion of different size nano micelles after anesthesia. CT scans were performed in 0.5h, 1.0h, 1.5h, 2.0h, 2.5h, 3.0h, 3.5h, 4.0h, 4.5h, 5.0h, and 5.5h after administration with application of GE Discovery HD750 CT GSI scanning mode. GE ADW4.5 workstation to measure the concentration of the polymer nano micelle in transplanted tumor, the dynamic evolution of the micelles was observed. Statistical analysis was used to analyze the difference of iodine content in each group. Results:1. The VX-2 rabbit tumor model was established in this study with success rate of 90%. The improved method was applied to block the incision with gelatin sponge and biogel, which could reduce the incidence of tumor growth and peritoneal implantation. Ultrasound examination will be implementing of rabbits on day 3, 6, 9,12,15,18 and 21 after implantation. The mean maximum diameter of the renal cell carcinoma was 5.6±0.9mm、8.8±1.8mm、12.1±1.8mm、15.5±1.9mm、18.3±2.6mm、25.2±3.7mm. Ultrasound characteristics are different on the different time points. When the tumor grew 15~18 days it would meet the experimental requirements, in this phase, the experimental rabbits were selected for the drug injection and CT scanning.2. There was no significant difference in rabbits age and tumor size between the three groups(P>0.05). After three groups were given the different sizes of polymer nano micelle intravenous injection, the content of iodine in renal cell carcinoma was higher than that in normal renal tissue, and changes over time showed accumulation retention effect of the tumor site. The content of iodine in group A was the highest after 3.0h:(1.44±0.26) ×100ug/cm3; in group B was the highest after 3.5h:(2.05±0.21) ×100ug/cm3; in group C, the highest iodine content appeared after 1.0h:(2.12±1.26) ×100ug/cm3. The iodine content of the tumor in different time points after the administration of three kinds of micelles was analyzed, and the difference was statistically significant(P<0.05). Conclusion:1. The improved method to establishing VX-2 renal cell carcinoma in rabbits by open surgery tumor block implantation is simple, the successful rate of tumor formation is high, reduced the incidence of ectopic implants and bleeding caused by surgery, and the biological properties of tumor are stable. It is an ideal method for making animal model of renal cell carcinoma. Ultrasound imaging is the first choice for the observation of tumor growth. The characteristics of different periods of tumor were different. Tumor bearing rabbits with tumor growth 15-18 days were in line with the common clinical features of renal cell carcinoma.2. Using polymer nano micelle with different sizes containing iodine to experiment in vivo animal models, application of energy spectrum CT technology to scan, the quantitative parameters of micelle concentration in VX-2 renal cell carcinoma can be obtained accurately. Different sizes of polymeric nano micelles in the tumor, its characteristics of concentration and the dynamic evolution of the law are different. Suggest that the complex microcirculation structure makes the EPR effect presented polymorphism. Study on the quantitative relationship between the concentration of the tumor and the polymer nanoparticles distribution after administration by EPR effect is valuable, the experimental result can provide a scientific basis for the design and clinical application of the polymer nano micelle carrier system. |
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