| Objectives To evaluate the effects and mechanism of different prescription medicine on acute gouty arthritis in mouse acute gouty arthritis model.Methods(1)Mouse acute gouty arthritis model was established by hypoxanthine fed,subcutaneous injection of oxonate and right ankle joint of sodium urate.(2)The efficacy was evaluated by ankle circumference difference,mice gait score,phosphotungstic acid reduction of serum uric acid levels in mice.(3)The level of IL-1β,TGF-β1 and URAT1 in serum were used to evaluate the mechanism of first prescription with ELISA.(4)The pathology of ankle abnormalities in differrent time was assessed by optical microscopy.Results(1)There were significantly different between experiment and control groups in ankle circumference difference,gait score and serum uric acid values in various times.Mice in experiment group occurred swelling in the joints,obvious limp and increaing serum uric acid levels after administration.Model mice ankle circumference difference,gait score and the presence of serum uric acid values were statistically significant(P<0.05)between the different sampling time and the normal control group,the mice showed significant swelling in the joints after modeling,obvious limp,serum uric acid levels continue to rise.(2)Reducing ankle swelling and improving claudication were obversed in mice treated with first prescription(P<0.05)on days 3.The effect of different prescription on ankle swelling and claudication was as follows:first prescription>third prescription>forth prescription>second prescription.First prescription and third prescription could significantly reduce the level of uric acid on 5 day(P<0.05).The effect of different prescription on eliminating uric acid was as follows:first prescription>third prescription>forth prescription>second prescription.(3)Reducing ankle swelling and improving claudication were obversed in mice treated with high dose first prescription(P<0.05)on days 5.The level of uric acid in serum was desclining persistently in first prescription on 3day(P<0.05).First prescription had a advantage over reducing uric acid tha in benzbromarone,One high-dose prescription drawn first five days can significantly reduce ankle swelling in mice,mice improve claudication(P<0.05);One prescription coverage in each dose group in the first three days begin sustained reductions in serum uric acid in mice levels(P<0.05),drawn in the first seven days of its role in lowering uric acid compared benzbromarone strong(P<0.05);and highly effective than low-dose group,middle dose group.(4)Serum IL-1β levels in the high-dose group on day 1 were significantly lower than with the model group(P<0.05);low dose and high dose levels of serum TGF-β1 in the first three days compared with the model group was significantly increased(P<0.05);high dose levels in serum URAT1 drawn first five days compared with model group was decreased significantly(P<0.05);Description first prescription could be elevated TGF-β1 levels and serum lower IL-1β levels to improve the degree of joint swelling of mice and gait score,down by URAT1 content to lower serum uric acid levels.(5)Synovial cell hyperplasia,interstitial edema,a large number of neutrophils and monocytes were occurred in first prescription group on days 3.There were large of necrotic tissue,a little amorphous material mixed and large of neutrophil infiltration in benzbromarone.Joint pathology in first prescription was significantly improved with interstitial edema,vascular dilatation and congestion,a small amount of mononuclear cell infiltration compared with the previous time on days 7.However,there were a lot of the joints of mice synovial cells,fibroblast proliferation,and a number of giant cells and a small amount of monocyte infiltration of neutrophils in benzbromarone.Conclusions(1)It was a successfully method to establish mouse acute gouty arthritis model through gavage oxonate subcutaneous injection of sodium urate and ankle.(2)There was significantly effect on owering serum uric acid levels,anti-inflammatory,analgesic aspects in all groups especially in first prescription.(3)The mechanism of inhitit acute gouty arthritis of first prescription probably suppressed inflammation by elevated serum levels of TGF-β1,reduced IL-1β levels and serum uric acid URAT1 level.One prescription treatment of acute gouty arthritis may be to suppress inflammation by elevated serum levels of TGF-β1 and reduced IL-1β levels,reduce joint pain;through down to lower levels of serum uric acid URAT1 level.(4)Arthropathy in all grous were imrpoved on days 7,meanwhlile,lymphocyte infiltration was obviously signficant in first prescription. |
PDF Full Text Request