Efficacy And Mechanism Of Bacillus Licheniformis On Experimental Colitis Related Liver Diseases

Inflammatory bowel disease(IBD) specifically refers unknown etiology and pathogenesis of inflammatory bowel disease(idiopathic inflammatory bowel disease), which includes ulcerative colitis(UC) and Crohn’s disease(CD). Now that its incidence may be genetic, immune, intestinal mucosal barrier dysfunction, diet, mental and other factors caused by the combined effects. Although IBD is mainly located in the intestinal lesions, but associated with a variety of intestinal manifestations is not uncommon, hepatobiliary disease is considered to be one of the most common intestinal manifestations. Recent studies have found that when IBD, intestinal flora resulting in increased mucosal permeability, intestinal barrier damage, bacterial translocation and endotoxin increased production, transported to the liver via the portal system. Endotoxin as the main pathogenic substances, which can trigger inflammatory cytokines(such as TNF, IL-1, IL-6, etc.) and co-stimulatory molecules massive release of direct damage to liver cells, eventually leading to the occurrence of liver and gallbladder disease. Bacillus genus of viable aerobic probiotic capsule formulation into the intestine while inhibiting the growth and reproduction of spoilage bacteria consume oxygen manufactured by the anaerobic environment, promote normal anaerobic bacteria such as Bifidobacterium, Lactobacillus and other reproduction, reduce bacteria endotoxin produced; and can reduce the intestinal pH, the acidic environment to promote the excretion of ammonia and endotoxin, which will help improve liver function.The experiment is aimed to investigate the therapeutic function and mechanism of experimental colitis associated hepatobiliary disease by Bacillus licheniformis. 45 male BALB / c mice were randomly divided into 3 groups consist of normal control group, DSS group, DSS + Bacillus licheniformis group. Configuration 3% dextran sulfate sodium(DSS) solution with sterile distilled water as the only drinking water for DSS group and DSS + Bacillus licheniformis group for 10 d, the normal control groupdrinking distilled water. Bacillus licheniformis(108CFU / ml) in modeling from the first day were administered orally, the other two groups to be normal saline as control(0.2ml / 10 g, 1 times / day). The mice were sacrificed according to the ethical requirements after 10 d, collected blood, colon and liver tissue.During the experiment to observe disease activity index score(DAI), colon length measurement, colon general morphology and histological index(HI). Using automatic biochemical analyzer serum alanine aminotransferase(ALT), aspartate aminotransferase(AST).To detect D- lactic acid, diamine oxidase(DAO), endotoxin, and the levels of TNF-α, IL-6 of liver tissue homogenate by enzyme linked immunosorbent assay(ELISA).Experimental results show that through the application of Bacillus licheniformis the DSS group, DSS + Bacillus licheniformis group DAI score, HI score, serum ALT, AST, D- lactate, DAO, endotoxin compared with the normal group were significantly higher(P <0.05), at the same time,the liver tissue in TNF-α, IL-6 were significantly increased(P <0.05). The DSS + Bacillus licheniformis group compared with DSS + group the DAI score, serum ALT,AST, D-lactate,DAO,endotoxin were decreased(P <0.05);the level of TNF-α, IL-6 of liver tissue homogenate were significantly lower(P <0.05).This study confirmed that Bacillus licheniformis has a therapeutic effect on experimental colitis and the related hepatobiliary disease, which suggested that it may be via regulating the intestinal flora, promote intestinal mucosal barrier function recovery, so that reducing bacterial translocation and endotoxin generation of the blood, thereby reducing the damage of endotoxin and inflammatory factors on the liver. Bacillus licheniformis can promote the recovery of intestinal mucosal barrier function, so that in the anti-colitis effect while improving liver function, reduced expression of inflammatory cytokines, promote immune function recovery, more conducive to the comprehensive treatment of UC.To give new ideas of identify UC intestinal manifestations, provide early and effective treatments, further control disease progression and improve the prognosis of UC.