Investigation Of The Infection Status Of Human Parvovirus B19 Among Unpaid Blood Donors In Chongqing

Background and objectives:We aimed to investigate the prevalence status of human parvovirus B19 among unpaid blood donors of Chongqing,thus providing the information for establishing detection process of B19 virus for unpaid blood donors in Chongqing.In this study,we studied the serum and molecular epidemiological situation through analyzing B19 IgG positive rate,B19 Ig M positive rate,double positive rate,double negative rate and B19 DNA positive rate,the correlation between the prevalence rate of B19 with gender,age,blood donation season,and the co-infection of B19 and infectious viruses related to blood transfusion.This study will provide the reference resources for screening work of unpaid donors,and provide information for establishing scientific and reasonable transfusion blood security policy in Chongqing,even in the whole nation.Methods:1.Collection of samplesAt least 50 blood samples per month,namely at least 150 blood samples were randomly selected among the qualified blood samples through the detection of human immunodeficiency virus(HIV),hepatitis B virus(HBV),hepatitis C virus(HBV),Treponema pallidum(TP),and alanine aminotransferase(ALT).At last,full-year 752 qualified blood samples and 385 unqualified blood samples were randomly collected.2.Serological detection of qualified blood samplesB19 IgG and B19 IgM antibodies among 752 qualified blood samples from unpaid donors were detected by enzyme linked immunosorbent(ELISA)method.And then B19 IgG positive rate,B19 IgM positive rate,double positive rate,double negative rate were analyzed.We also obtained the information about the prevalence rate of B19 among unpaid blood donors in Chongqing,meanwhile,we analyzed the correlation between the positive rate of B19 Ig G and B19 Ig M with gender,age,career,blood type,residence,blood donation season according to the results of serologic detection.3.Serological detection of unqualified blood samplesB19 IgG and B19 Ig M antibodies among 385 unqualified blood samples from unpaid donors were detected by enzyme linked immunosorbent(ELISA)method.And then B19 IgG positive rate,B19 Ig M positive rate,double positive rate and double negative rate were analyzed.At the same time,we analyzed the co-infection of B19 virus and HIV,HBV,or HCV,and surveyed the correlation between them.4.Molecular biological detectionThe DNA of 752 qualified blood samples were extracted,and amplified using the ABI PRISM 7900 real-time fluorescent quantitative PCR instrument,then the results were analyzed and the positive rate of B19 DNA among unpaid blood donors were obtained.Results:1.Among the 752 qualified unpaid blood samples,the positive rate of B19 IgG was 36.7% and the positive rate of B19 IgM was 5.59%,there was statistic difference between them(P<0.001).There were 14 double positive samples(1.86%)and 448 double negative samples(59.57%).In addition,the positive rate of B19 Ig M in female donors was higher than that in male donors,and there was statistic difference((P<0.05).Meanwhile,we found that the age and residence are the influence factors of B19 infection,the infection rates from 35 to 45 and from 46 to 55 years old people were higher than 18 to 25 years old people(P=0.000).Furthermore,the infection rate among people in county was higher than urban area.2.Among the 385 unqualified unpaid blood samples,the positive rate of B19 Ig G was38.18%,o and the positive rate of B19 Ig M was 5.19%,there was statistic difference between them(P<0.001).There were 11 double positive samples(2.86%)and 229 double negative samples(59.48%).Moreover,the positive rate of B19 Ig M in female donors was higher than that in male donors,and there was statistic difference((P<0.05).Meanwhile,we found that the positive rate of B19 lgG was increased with age(P<0.05),and increased with decrease of educational level(P<0.05),and there was statistic difference of B19 lg G positive rates between urban and suburban donors(P<0.05).Among HIV-positive samples,there were 29.91% B19 Ig G-positive samples,6.54% B19 Ig M-positive samples and 5.61% double positive samples.Among HBV-positive samples,there were 36.92% B19 IgG-positive samples,6.15% B19 Ig M-positive samples and 3.85% double positive samples.Among HCV-positive samples,there were 43.97% B19 Ig G-positive samples,2.59% B19 IgM-positive samples and there was on double positive sample.3.The results of real-time PCR showed that the 3 B19 DNA-positive samples were detected from 752 qualified blood samples and the positive rate was 0.04%.Conclusions:1.There were a few people with previous infection and acute infection among qualified blood donors in Chongqing.Although the prevalence rate of B19 in Chongqing was on a lower than other cities,there was still risk of infecting the virus by blood trasfusion.In the case of undevelopment of the screening work of B19 for unpaid blood donors,the furher studies are needed to clarify the risk of B19 infection by blood transfusion.2.The analysis results of co-infection of B19 virus and other infection viruses related to blood transfusion,such as HIV,HBV and HCV,showed that the people with lower immunity low were susceptible to B19 virus.3.In this study,we investigated the molecular epidemiological situation of B19 among unpaid blood donors in Chongqing for the first time,the results showed that there were still a few B19-positive people among qualified unpaid blood donors in Chognqing,but it showed the low serum B19 DNA titers and prevalence rate.4.At present,the screening work of B19 for blood and blood products has not been carried out,the results in this study will be information for establishment of blood screening strategy.