Expreesion Of IgE And Substance P In The Organs Of Guinea Pigs Which Died In Allergic Shock,and Its Significance In Forensic Medicine

Objective: By establishing a cavy model of allergic shock, testing the content of Ig E in cavy serums, observing the expression of Ig E and Substance P(SP)in cavy tracheas, lungs, stomachs, spleens and other tissues in the immunohistochemical staining method, it is appropriate to determine the value of Ig E and SP in the diagnosis on the death of allergicshock, so as to provide a basis for the pathological morphology.Methods: 33 healthy adult cavies were divided into two groups. 24 were in the experimental group and 9 were in the control group. Allergens(a mixture of human serum)were injected into the right hind paws of the cavies in experimental group. After three weeks, being injected again and induced allergic shock, they were observed the performance of the injection. The cavies in control group were injected with normal saline and executed. After their death, theblood was extracted for treatment and the serum Ig E value was detected in ELISA method at the different time points(0h, 48 h, 7D). The conventional HE staining and histologicalexaminations were used to anatomize their carcasses and fix their tracheas, lungs, stomachs, spleens and other organs. And the immunohistochemical staining method was used to observe the expression of the Ig E and SP in the organs at the different time points(0h, 48 h, 7D). SPSS19.0 software was used to do a statistical process in obtained data.Results:(1)The cavies in the experimental group showed different degrees reflection of allergic shock in one minutes after re injection and 18 of them died in 30 minutes. However, the control group did not change.(2)The anatomy of carcasses which died in different periods in experimental group results showed laryngeal edema, dilatation of heart reaction and other reflection. Visceral congestion, eosinophilic infiltration and other manifestations were observed by microscopic examination. There was no discovery in these areas.(3)The Ig E and substance P in the experimental group were detected by Immunohistochemical method, and they were significantly higher than that in the control group, P<0.01.There was no difference in the expression of the Ig E and substance P at the different time points(0h, 48 h, 7D)(4)The expression of Ig E in serum was detected by ELISA. The expression of the experimental group was found to have a significant difference with the control group, P<0.01.And there were not significant in experimental group at the different time points(0h, 48 h, 7D), P > 0.05.Conclusion:(1)The establishment was successful in a cavy model of allergic shock in multiple humanserums.(2)Ig E content in serum and the content of Ig E and substance P in tracheas, lungs, stomachs, and spleens were obviously increasing in the death of cavies with allergic shock.(3)Using immunohistochemical staining method to detect Ig E, P content, it can be expected to provide a certain basis for the death of allergic shock in the forensic identification.