Research On Hypoglycemic Activity And Mechanism Of Physalis Alkekengi Extract

Diabetes mellitus(DM)is a serious global public health problem,not only do great harm to people’s health,also is the third largest non-communicable diseases after the disease of cardiocerebrovacular and cancer.Although many drugs were used to treat DM,but most of this has side effects,so to find low toxicity and efficient new antidiabetic drug is the enormity of the task to medical researchers.Physalis alkekengi is a kind of medicine food homology of Traditional Chinese Medicine(TCM),which rich in steroid compounds,alkaloids,amino acid and other chemical.Physalis alkekengi has shown various pharmacological and physiological effects such as anti-inflammatory,antioxidant,anti-fungal,hypoglycemic,febricide,expectorant,diuresis,and so on.However,the antidiabetic effects of Physalis alkekengi are not clear.The current study aimed to investigate its antidiabetic potential and the possible underlying mechanisms in mature adipocyte cells and HepG2-GFP-CYP2E1(E47)cells.The main content of this search could be divided into the three following parts:1.Dry ground and fruit of Physalis alkekengi(ground 70 g and fruit 10 g)were extracted three times with 2.5 L of 75%of ethanol at 60 ℃.The residues were re-suspended in an appropriate volume of deionized water and successively extracted with an equivalent volume of petroleum ether(PE)and ethyl acetate(EA),giving six fractions for Physalis alkekengi extract(PE of ground,EA of ground,aqueous extract(AQ)of ground,PE of fruit,EA of fruit,and AQ of fruit).Then evaluate the antioxidant activity and antidiabete activity through FRAP assay,ABTS assay,the total phenolic content and a-glucosidase inhibition assay.The ethyl acetate extract(EA)of Physalis alkekengi ground/fruit possesses a strong antioxidant activity,which supports the higher total phenolic content of different extracts.Notably,the EA ground/fruit extract exhibited a significant inhibition of a-glucosidase capacity.2.HPLC-ESI-QTOF-MS analysis identified the components of EA ground/fruit as mainly flavonoids,phenolic acids and other components.It offers promising antidiabetic effects may be mainly attributed to its potent antioxidant potential.3.The EA of Physalis alkekengi ground/fruit decreased the reactive oxygen species(ROS),lactate dehydrogenase(LDH)and malondialdehyde(MDA)levels and increased the catalase(CAT)levels by H2O2-induced oxidative damage in 3T3-L1 pre-adipocyte cells and by C YP2E1-induced oxidative damage in HepG2-GFP-CYP2E1(E47)cells.Mechanically,we found that the CYP2E1 mRNA and protein levels in E47 cells significantly decreased in a dose-dependent manner,while the GLUT4 mRNA and protein levels in mature 3T3-L1 adipocytes increased.Based on this evidence,Physalis alkekengi extract displaying antidiabetic activity might enhance GLUT4 expression and function by inhibiting the expression of CYP2E1 mRNA and protein,and this activity might be due to a high concentration of chemical compounds,especially flavonoids and phenolic acids.This study investigates the possibility of Physalis alkekengi,including its ground and fruit,as a strong candidate antidiabetic herbal medicine or antidiabetic dietary supplement.