The Expression Of Caspase-3 After Cerclage-induced Spinal Cord Injury In MP Rats

Objective: Through the original ring ligation constructing rat models of acute spinal cord injury and in the early use of intravenous methylprednisolone,rat motion state,pathology and observation Caspase-3 expression situation in different time period observation,SCI early drug treatment strategies are discussed,and to provide useful help in clinical work.Methods: The successful establishment of the rat ring ligation method in the preliminary work,through a large number of animal experiments can prove that this method is stable and repeatable,and the method is simple.Specific methods for randomly selected 115 SD male rats,rats aged about 13 weeks,were randomly divided into for three groups: group A（control group）underwent laminectomy without cerclage treatment;group B（control group）: surgical laminectomy and postoperative 6h spinal cord cerclage;C group（experimental group）: surgical laminectomy and postoperative 6H spinal cord cerclage,and in 3 hours after intravenous injection of MP.Ring ligation method of rat spinal cord injury model induced specific construction method that preoperative first use mobile C-arm machine filming location of rat lumbar spine,select rats T10¹1 parts,skin preparation in preparation for the area of the operation,and disinfection with Iodophor conventional shop towel operation.Wash your hands after disinfection along the rat dorsal midline incision in the calibration area selected a length of about 3cm,and gradually cut the skin,subcutaneous fascia,and vertebral body parts on both sides along the longitudinal through muscle tissue,through minimally invasive surgical instruments gradually bite except the lamina exposed dural sac,completely exposed after surgery using absorbable suture through the operation of micro needle through the spinal cord of the rat spinal cord,measured diameter of C1,and then through the calculation formula,which calculate the circumference of C2=C1*0.7^1/2C2 value,the C2 value even if the required degree of spinal cord compression caused by ligation ligation required in the model,after the completion of the 1 days,3 days,7 days,14 days and 21 days respectively to detect the motor function of rats,including plate rats and BBB score,evaluation after the rats were sacrificed and the spinal cord removed cerclage pathological tissue sections of HE staining,immunohistochemistry and in situ end End of deoxyribonucleic acid（DNA）transfer enzyme mediated deoxyuridine triphosphate（d UTP）labeling（TUNEL）examination,comprehensive data after the data were statistically analyzed.Corresponding conclusions.Results: the experimental results show that the A group in the blank control group,the array of oblique plate score,BBB score,HE staining and immunohistochemistry,the number of Caspase-3 positive cells showed no obvious abnormalities.And compared with the A group,the B group in the experimental group and the BBB score was significantly lower than the A group,while the C group compared with the experimental group was lower than the blank control group,but higher than the B group.And number of positive cells in the Caspase-3 between group B and group C expression number is higher than that of the control group,between group B and group C compared,peak expression appeared in 3 days,from 7 days began to gradually decline,but group B the number of positive cells in each group were higher than C group.All the data were statistically significant（P < 0.05）.Conclusion: 1 original ring ligation induced rat spinal cord injury model is simple and effective,repeatable,and can cause spinal cord compression static,the influence factors are less,can provide an alternative method for similar research;resulting in 2 acute spinal cord injury injury is a continuous process,the main damage is the primary injury to spinal cord injury and oppression,the latter is mainly the process of apoptosis,caspase-3 also called death protease is a thought in the process of apoptosis is the most important proteins,of which Caspase-3 is one of the most important one,is the downstream effects of multiple apoptotic pathways in common part is apoptosis ELISA3 the only way which must be passed through the animal experiment to observe;visible,MP treatment early after onset of SCI rats in exercise The recovery and the recovery of the pathological state have different degrees of promoting effect,which provides theoretical evidence for the clinical use of MP.