The Ethology Effect Of Treating Acute Spinal Cord Injury In The Rat Model With Tetramethylpyrazine

Objective To compare nerve function recovery of treating acute spinal cord injury model by the tramethylpyrazine (TMP) and methylprednisolone (MP). Further explore mechanism of action of TMP on acute spinal cord injury.Materials and Methods 30 adult male SD rats were randomly divided into three groups:MP treatment group (group A, n=10), TMP treatment group (group B, n= 10), the control group (group C, n= 10). Moderate spinal cord injury at T10 level model was maken by modified Allens's weight drop method.30mg/kg methylprednisolone was used to group A intraperitoneally at 15 minutes after surgery, at fellowing 23 hours after surgery,5.4 mg/kg methylprednisolone was used to group A intraperitoneally with 4 times.200mg/kg of TMP was used to the group B intraperitoneally and the group C were treated with the same volume of saline vehicle (NS) 30 minutes after sugery. Each model was given the same dosage once a day until euthanasia 5 days postoperative. To evaluate SD rat actue spina cord injury model behavioral score with modified inclined plane test and Rivlin BBB (Basso, Beattie, Bresnahan) score at after 1 day,3 days,7 days,14 days,21 days postoperative, respectively. Data was analyzed with SPSS 13.0 statistical analysis software.Results The mean ramps critical angle degree in all the three groups of rats increased gradually with time postoperative.The critical angle degree of high ramps in both group A and B was higher compared with group C (P< 0.05) at 7,14, and 21 days postoperative, however, there was no significant difference between goup A and B (P>0.05). The mean BBB score in the three groups increased gradually postoperatively too, The mean BBB score in both group A and B was significant higher compared with group C (P< 0.05) at 7,14, and 21 days postoperative, however, there was no significant difference between goup A and B (P>0.05).Conclusion:The effect of treatment in the early phase of acute spinal cord injury with methylprednisolone is significant, and prior to the TMP. The TMP can be used to treat acute spinal cord injury.