Influence Of Flavonoids And B Vitamins On The Lysozyme Secondary Structures And Activities By Spectral Methods

Lysozyme(LYSO) could dissolve the bacterial cell wall, first discovered by Fleming in human secretions, which has the anti-bacterial, anti-inflammatory, antivirus and antitumor effects. Flavonoids are important natural organic compound, exhibiting a wide range of biological activity, which are widely used in traditional medicine because of the anti-bacterial activity. Both LYSO and flavonoids have the anti-bacterial effects, but the anti-bacterial ability is limited when they are used alone, thus it is very necessary to explore whether they can combine to use or not. The research about the influence of flavonoids on the LYSO secondary structure and biological activity may provide an important theoretical significance for searching the new anti-bacterial way. B vitamins may play the role in cooperating with LYSO in terms of anti-bacterial effects. It can provide the theory evidence about the combined utilization of antibacterials by studying the effect of B vitamins on the LYSO secondary structure and inferring the influence of biological activity and function.Objective: We studied the influence of flavonoids on the LYSO secondary structure and biological activity, and the change of LYSO secondary structure in the presence of B vitamins.Methods: The influence of flavonoids on the LYSO α-helix structure was inferred by the CD and Raman methods. We studied the LYSO α-helix relative contents before and after adding flavonoids whether had the significant difference by the ATR-FTIR method with the Origin 9.0 and SPSS 13.0 software, inferring the change situatioan of LYSO active site in combination with the CD and Raman methods. The LYSO activity was measured by the UV-vis spectroscopy as the assay kit instrument book. The change of LYSO secondary structure in the absence and presence of B vitamins was studied mainly by the ATR-FTIR method, inferring LYSO biological activity and function change situation.Results: The positions and intensities of LYSO spectra changed after adding luteolin, luteoloside, Baicalein, baicalin, wogonin, quercetin, quercitrin and kaempferide observed by the CD and Raman methods. The LYSO secondary structure relative contents changed in the presence of the above flavonoids. The LYSO α-helix relative content had the significant difference before and after adding luteolinluteoloside, baicalin, wogonin, quercetin and kaempferied. The LYSO activity decreased with the addition of the above eight flavonoids, which changed obviously in the presence of luteolin, luteoloside, baicalin, wogonin, quercetin and kaempferied. LYSO α-helix relative content had the significant difference before and after adding luteolin, quercitrin and VB1 or VB3; baicalin, kaempferide, quercetin and VB1, VB3, VB5; luteoloside and VB1; wogonin and VB3. The LYSO α-helix relative content had no significant difference in the absence and presence of baicalein and VB1, VB3, VB5.Conclusions: The flavonoids might affect the LYSO α-helix structure inferred by the CD and Raman methods. The LYSO secondary structure relative contents changed after adding the flavomoids, which indicated the above eight flavonoids had the effect on the LYSO secondary structure, confirming the above conjectures. The ATR-FTIR results also indicated the LYSO active site might be influenced with luteolin, luteoloside, baicalin, wogonin, quercetin and kaempferied obviously. The LYSO activity experiments indicated luteolin, luteoloside, baicalin, wogonin, quercetin and kaempferied affected the LYSO activity obviously, which were in accordance with the conjectures about the change of active site. The biological activity and function of LYSO might be changed after adding luteolin, quercitrin and VB1 or VB3; baicalin, kaempferide, quercetin and VB1, VB3, VB5; luteoloside and VB1; wogonin and VB3. The LYSO biological activity and function could be changed little when baicalein and VB1, VB3, VB5 were added synchronously.