Simultaneous Determination Of Thirteen Antidiabetic Drugs By HPLC-MS/MS

High performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) is a modern analysis technique, which combine a high separation ability of high liquid chromatography (HPLC) with high identification ability of mass spectrometry. It has the advantages of high accuracy, high precision, high reproducibility, detecting multicomponent in short time and so on.The high incidence of diabetes causes more and more use of western medicine antidiabetic drugs, and the harm of drug overdose to human body is quite serious. Accurate the content determination of drug not only play an important role to the doctor’s treatment, but also to control the misuse of western medicine on the market situation.In this paper, the liquid chromatography tandem mass spectrometry and antidiabetic drugs are recommended, using high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) has established the one-off fast and efficient method to measure the content of 13 antidiabetic drugs at the same time. Using solid phase extraction method (SPE) for sample pretreatment, to remove impurity effect is very good. Choosing Agilent ZORBAX Eclipse Plus C18 (150 mm x 4.6 mm, including 5 μm) chromatographic column to separate 13 antidiabetic drugs. The mobile phase is consisted of acetonitrile-0.1% acetic acid solution (with ammonia adjust PH 3.5). Using multiple reaction monitoring (MRM) mode to quantify the Tolbutamide、Tolazamide、Glibenclamide、Glipizide、Gliclazide、 Gliquidone、Glimepiride、Phenformin、Metformin、Rosiglitazone、Pioglitazone、Repaglinide、 Nateglinide. This method is high selectivity and high sensitivity.