The Analysis Between Aggressive Phenotype And Genotype In Turpan Gamecocks

Objective: Discussing and exploring the genetic characteristic of aggressive phenotype and genotype of Turpan Gamecock by “The analysis between aggressive phe n-otype and genotype in Turpan Gamecocks”, searching molecular marker of aggressi v-e behavior,applying molecular marker assisted selection to the seed selection of T-urpan Gamecock, and laying the foundation for aggressive molecular breeding of Tu r-pan Gamecock.Methods: In this study, Turpan Gamecock which have strong aggressiveness hy bridized with New Roman hens which is weak in aggresiveness to generate F1 generation. Next detected the expression of SLC6A4 and NR2E1 of Turpan Gamecock, N-ew Roman egg breed cockerel and F1 generation in six months from hatched to sexual maturity by real-time quantitative fluorescence PCR technology. Then used the acquired F1 generation to make full-sib mating and generated the F2 genaration and collected blood samples from parents and F1,F2 generation chicken,and used PCR-SSCP to detected the SNP polymorphisms in all exons and partial intron of SLC6A4 gene and NR2E1. Finally detected the aggressiveness in Turpan Gamecock and hybr-id progeny by mirror fight test and pairing fight test and through the correlation an-alysis between genotpye and phenotype to provide more accurate evidence for the r-elationship between genes and aggressiveness.Results:(1) Using real-time quantitive fluorescence PCR detected the expression of SLC6A4 in Turpan Gamecock, New Roman egg breed cockerel and F1 generation cock,from 0 month to 6 months,and found in the 7 phases,the developed trend lik-e wave pattern. The expression of SLC6A4 of brain is higher in 0 month,3months a-nd 6 months. In different phase,the expression of SLC6A4 in different chicken is d-ifferent. the expression in strong aggressive Turpan Gamecock is highest. The expr-ession of NR2E1 is detected in Turpan Gamecock, New Roman egg breed cockerel an-d F1 generation cock,from 0 month to 6 months,and foundthe developed trend is excalating in the 7 phases. The expression of NR2E1 of brain is higher in 6 months.In different phase,the expression of SLC6A4 in different chicken is different. the e-xpression in strong aggressive Turpan Gamecock is lowest. Which show that SLC6A4 and NR2E1 have associate with the aggressive behavior,and could lay the theoret-ical foundation for the SNP polymorphism of SLC6A4 and NR2E1, aims to obtain t-he molecular markers of SLC6A4 and NR2E1,and provide a reference for Molecule-r Marker Assisted Selection of Turpan Gamecock.(2)We found two SNPs polymorphisms in exon one of SLC6A4 which are C6213750 G and T6213768 G, base mutations and invoke change of amino acid. This chan-ge may be associated with aggressive behavior, because the genotype in the SNP lo-ci of exon one in Turpan Gamecock and New Roman hens were significantly differ-ent. The SNP polymorphism NR2E1 was detected and have not relationship with a-ggresive behavior.(3)In mirror fight test,Turpan Gamecock is extremely significantly higher than F1 generation in Fighting(P < 0.01);But the two breed are no signigicant difference in Non-fighting.In pairing fight test, Turpan Gamecock is significantly higher than F1 g-eneration in Fighting(P < 0.05);But the two breed are no signigicant difference in Non-fighting.(4)Through the correlation analysis between the exon one genotype of SLC6A4 and the phenotype tested by mirror fighting and pairing fighting. We found the BB genotype is significantly higher than the other genotype chicken, which show the BB genotype in exon one of SLC6A4 is close relation to the aggressive behavior.Conclusion: The expression of SLC6A4 is positive correlation with aggressive behavior in different chicken,and the expression of NR2E1 is negtive correlation with aggresive behavior in different chicken.Then we found one SNP molecular mark in SLC6A4, which is related aggresive behavior. we found significant different aggressi-ve behavior in Turpan Gamecock and F1 generation by the phenotypic analysis, and BB genotype in exon one of SLC6A4 is close relation to the aggressive behavior,and could to be reference genotype of Molecular Marker Assisted Selection of Turpan Gamecock.