Screening Of The Microbial Flooding Strains And Analysis Of The Metabolite

In this paper,the microbial strains were s creen ed and t he metabol ite was analyzed.The main purposes are scree ning high efficient st rains adaption to Ba olige Oilfield microbial flooding accordi ng t o the field s ituation,qual itative and quantitative analyz ing the strains m etabolit e and making defini te the microbi al flooding mechanism in order to provi de t heoreti cal guidanc e and technical support for large-scale application of microbial flooding in Huabei Oilfi eld.12 high efficient st rains were selecte d bas ed on t he development contradiction and reservoir condi tions of Ba oli ge oi lfield.The m icrobial optimization methods including culture m edium s creening,facultative anaer obic enrich ing,visbreaking and emulsi fication effect of strains,m etabolit e anal yz ing,degradation effect to oil,displ acement char acteristics through physica l si mulation were studied sys tematically in house tests.In order to implement integral m icrobial flooding in the 4 faul t blocks of Baolige oilfield,studi es on strains combination were conduted and finally 6 strains of HB1~HB6 were selected and appl ied in the field.Combining with indoor experiments and fiel d application of microbial floodi ng,the m onitoring methods of main metabolites including biosurfactants,bioga s and organic acid were est abli shed and studi ed quantitatively.The res ults showed t hat 12 strains can not onl y adapt to the reser voir environment,but also degrade the heavy components in the crude oil and present good oil displacement performance.The oil recove ry could be i ncreased by 15% according to the physical analog ex perim ents.The organic acid was prim arily acetic acid and crude oi l could absorb t he surfactants,production i ncreasing effect was significa nt when 3 g/L lipopeptide surfac ant was i njected int o the formation.The gas producti on rate was increased apparently after microbi alflooding and carbon dioxi de and nitroge n occupied a l arge proport ion in the produce d ga s.The increase of the gas producti on of can effectively enlarge t he formation pres sure,reduce oi l viscos ity and enhance oil recovery.