The Study Of EMT Promoted By RAGE In The Tumor Tissue Of Conlon Rectum Cancer (CRC) Patients Complicated With Diabets

Objective:To observe the expression of relative protein in the tissue of colon rectum cancer(CRC) patients with diabets(Db) in EMT progress. Medthods:Collecting some tissues, including the tumor tissues of CRC patients combining with diabets(Db)and nontumorous tissues. All the tissues were divided into control group、tumor group with Db and tumor group without Db. Associated protein RAGE、AGE、E-cad、β-catenin、and vimentin were detected by immunohistochemical staining; Western blot tested the levels of these protein RESULT:RAGE、AGE、E-cad、and vimentin were mainly located in cell membrane, β-catenin was mainly located in cytoplasm, and the color of two tumor groups deeper than normal group(P<0.01).The relative level of AGE、RAGE、β-catenin and vimentin in two tumor groups were higher than those in control group, E-cad was lower than it(P<0.01). Conclusion:the relative protein RAGE 、 AGE 、 β-catenin and vimentin are up-regulated, E-cad is down-regulated in the tumor tissue of CRC patients with Db. The EMT progress is promoted and it has played an play a vital role in enhancement to the development of CRC.