Expressions Of Ki67 And VEGF In Human Cervical Squamous Cell Carcinoma

Introduction: Cervical cancer has been considered as the most common malignant tumor of the female genital tract.According to statistics, the incidence rate of cervical cancer in the world of female malignant tumor is third. The incidence rate of cervical cancer in developing countries is just lower than breast cancer,The annual new cervical cancer patients in China are about 135 thousand, accounting for the one third patients around the world. In recent years, because of further development of gynecological surgery and medical technical level, the treatment effect of cervical cancer has made rapid progress, especially in patients with early stage that can be cured by surgery or radiotherapy, but the the incidence is increased significantly, and the patients with cervical cancer are more and more younger, also, the survival rate of 5-years of invasive cervical cancer is still low. Poor prognostic factors for early stage cervical cancer include large tumour diameter, pelvic lymph node metastasis, parametrial invasion, positive surgical margins and deep stromal and lymphovascular invasion. However, whether such prognostic factors are sufficiently accurate to estimate prognosis and determine therapeutic strategies remains controversial. Therefore, biological characteristics of cervical cancer should be understood and novel molecular markers should be developed to accurately predict the prognosis of patients.The growth, survival, and metastasis of tumor are depend on angiogenesis to provide adequate nutrition and oxygen. Without new vascular, the diameter of tumor will be limited in about 1-2mm, that is dormant state, if lack of capillary, thanatosis and apoptosis are easily happened in planted tumor. Vascular not only promote the proliferation of vascular endothelial cells, permeability of vascular,the formation of a vascular support, but also inhibit the apoptosis of tumor cells. The current study found that VEGF regulates angiogenesis through multiple signal pathways.As a marker for evaluation of cell proliferation, Ki67 is a kind of nuclear protein, that exists in proliferating cell nuclear.The genesis, invasion, planting and translation of tumor are closely related to cell proliferation. Ki67 plays an important role in the study of the biological behavior, the evaluation of cell proliferation of tumor. Even though Ki67 has been widely used to evaluate the growth of cells, the exact mechanism of Ki67 and its related genes, cell factors are still not clear.Aims: To investigate the expression of VEGF and Ki67 in cervical squamous cell carcinoma(SCC) and normal cervical tissues and their prognostic significance. Analysis the ralationship of VEGF and Ki67 with its clinical pathological parameters including age, FIGO stage, histological type, depth of tumor invasion, tumor differentiation, pelvic lymph node metastasis and vascular invasion in cervical cancer, so as to clarify whether they are interact and jointly promote the occurrence and development of cervical cancer, to provide a guidance in the treatment,Evaluation of efficacy,judgmentof prognosis in cervical cancer.Methods: Immunohistochemistry was performed to analyze the VEGF and Ki67 expressions in 120 cervical cancer tissues and 60 normal cervical tissues. Spearmen test was used to analyze correlation between protein expressions, χ2 exact tests were used to compare different protein expression rates.Results:1.VEGF immunoreactivity was positively expressed in cervical cancer cells of 67.5%(81/120)and normal cervical epithelial cells of 13.3%(8/60) of patients.Ki67 was positively expressed in 83.3%(20/120)and 28.3%(17/60)of cervical cancer and normal cervical tissues. The positive expression rate of VEGF and Ki67 was significantly increased in cervical cancer tissues compared with that in normal cervical tissues(p<0.05). 2.VEGF expression is positively associated to Ki67 expression. VEGF expression is related to vascular invasion( p =0.018) and pelvic lymph node metastasis( p = 0.013);VEGF expression has no relationship with age, FIGO stage, histological type, depth of invasion, tumor type in cervical cancer tissue(p > 0.05); Ki67 expression is related to FIGO staging(p = 0.027), vascular invasion(p = 0.018)and pelvic lymph node metastasis(p= 0.013); Ki67 expression has no relationship with age, histological type, depth of invasion, tumor type in cervical cancer tissue(p > 0.05).3.The expression of VEGF and Ki67 was significantly positively correlated(p=0.023).Conclusion:The expression of VEGF and Ki67 was significantly increased in cervical cancer. The expression of VEGF was positively correlated with the pelvic lymph node metastasis and vascular invasion; the expression of Ki67 was positively correlated with FIGO staging, vascular invasion and pelvic lymph node metastasis.The expression of VEGF and Ki67 was significantly positively correlated.