| Objective:This experiment using flow cytometry technique to detect different TNM staging colorectal cancer patients serum circulating tumor cells, analysis with the infiltration depth, lymph node metastasis and TNM stages, the relationship between the degree of tumor differentiation; Using ELISA method to detect different TNM staging of colorectal cancer patients serum interleukin 8 expression level, analysis of different TNM staging CXCL8 expression in serum in patients with colorectal cancer, in order to explore detection of colorectal cancer patients with peripheral blood circulating tumor cells (circulating tumor cells, CTCs) and the relationship between the expression of interleukin 8 and clinical value, and discuss the possible mechanism.Methods:1.Gathering on April 1,2015 to April 1,2016, kunming medical university third hospital,71 cases of colorectal cancer in colorectal surgery in patients with peripheral blood 10 ml, gathering on June 1,2015-2015, July 1 JCP 10 cases of healthy volunteers peripheral blood 10 ml as control group.2. The use of peripheral blood lymphocyte separation method of preparation of peripheral blood mononuclear cell suspension, split the test tube and to take care of, test tube mark CK19 were respectively, CD45 monoclonal antibodies. Negative to take care of the same type are control antibody.3. Having marked good peripheral blood mononuclear cells by flow cytometry, detect CK19+/CD45- cell, as a symbol of CTCs in patients with colorectal cancer.4. Using enzyme-linked immunosorbent (ELISA) method for the determination of serum CXCL8 content.5. The results with SPSS 13.0 statistical software package processing, the experimental results using statistical analysis, chi-square test with P< 0.05 for the difference was statistically significant.Results:1.33 cases were detected in 71 patients with colorectal cancer CTCs, CTCs positive rate of 46.48%, not detect CTCs in 10 cases of healthy volunteers, the positive rate of similar between the two groups have statistical significance (P< 0.05). 2.71 cases of colorectal cancer patients according to clinical TNM staging (â… , â…¡, â…¢ and IV), CTCs positive rate were 7.69%, 36.84%, 52.00% and 7.69% respectively, the four groups of positive difference was statistically significant (P< 0.05); According to lymph node metastasis status (such as NO, N1, and N2), CTCs positive rate were 24.00%,48.14%,73.68%, three sets of positive difference was statistically significant (P< 0.05); According to the clinical tumor infiltration depth (T1, T2, T3, T4), CTCs in patients with positive rate were 20.00%, 25.92%, 44.00%, 78.57%, four groups were statistically significant difference (P< 0.05). According to the differentiation degree of tumor differentiation of (high, medium and low differentiation), CTCs in patients with positive rate were 18.75%, 40.00%, 65.00%, three groups were statistically significant difference (P< 0.05).3. colorectal cancer patients serum CXCL8 level increased obviously, and significant difference was found in normal control group.4. colorectal malignant degree is higher, the worse of differentiation, and elevated serum CXCL8 levels, significant difference was found in high differentiated and poorly differentiated groups; Colorectal cancer with distant metastasis serum CXCL8 level differences between the groups with and without distant metastases.Conclusion:1. The application of flow cytometry detection technology, peripheral blood CTCs can detect colorectal cancer patients.2. The late colorectal cancer patients with clinical stage, tumor infiltrating the more depth, lymph node metastasis, tumor differentiation, the worse the CTCs detection rate is higher; TNM staging were positively correlated with serum CXCL8 content, namely with TNM staging change, the patients serum CXCL8 expression gradually increases, its high expression is an independent risk factor for patients with poor prognosis.3. Colorectal cancer patients serum CXCL8 content is significantly higher than healthy group, the difference is statistically significant.4. Different TNM staging CXCL8 content in serum in patients with colorectal cancer and the number of circulating tumor cells as a positive correlation. |
PDF Full Text Request