Soluble CD147 Involved In The Regulation Of HCC Invasion By Active Akt Pathway

Background:Hepatocellular carcinoma(HCC) is one of the most common cancer in worldwide. Because of its highlyprogressive characteristics(i.e. rapid progression, poor response tomany therapies and high risk of relapse), HCC has becomeanincreasingthreat tohuman health.CD147 is a kind of type I transmembrane glycoprotein which is highly expressed in HCC cells.Recent research hasindicated that CD147 is closely associated with the initiation HCC mediated by hepatitis B(HBV) and hepatitis C(HCV). Moreover, CD147 can involved in the progress of HCC by induce the expression of matrix metalloproteinases(MMPs), increase tumor angiogenesis and tumor cell proliferation, reduce tumor cell apoptosis and induce multi-drug resistance. The aim of is study is investigated the pathway involved in the regulation of invasive ability of hepatocellular carcinoma(HCC) cells mediated by soluble CD147, andvalid these finding in HCC patients.Methods: We explored the role of soluble CD147 on MMPs secretion by Western blot. Invasion assay be applied to HCC cells, to investigate the effects of soluble CD147 on invasive ability regulation. Serum CD147 levels were detected by the enzyme-linked immunosorbent assay. Tissues CD147, MMP-9 and p-Akt expression were assayed by Immunohistochemistry.Results: Soluble CD147 regulate invasive ability of HCC cells by associated with surface CD147 and induce the activation of Akt-related pathways. The concentration of soluble CD147 in serum of patients with HCC was significantly elevated compared with healthy controls.(P < 0.01) Serum soluble CD147 levels were positively related with the expression of surface CD147, MMP-9 and p-Akt in tumor tissues.(P < 0.01)Conclusions: Soluble CD147 regulate the invasive ability of HCC cells by affect Akt-related pathways and, then, induce the expression of MMP-9. Serum soluble CD147 is a potential diagnostic and prognostic biomarker for HCC.