Expression And Clinical Significance Of TopoⅡα And Ki67 In Invasive Breast Cancer Tissues

Objective The study the detected the expression of topoisomeraseⅡalpha and Ki67,and analyzed the correlation between the expression of TopoⅡα and Ki67 with the clinical and pathological characteristics in invasive breast cancer to investigate the role of TopoⅡα and Ki67 positive expression in mechanism of invasive breast cancer’s occurance,development and metastasis.Methods The 78 breast cancer patients who accepted surgical treatment were retrospectively studied and tested by immunohistochemistry(IHC) staining. Compare the relationship between the expression of topoisomeraseⅡα and Ki67 with the clinical and pathological characteristics such as age,tumor size, lymph node metastasis,the expressions of Estrogen Receptor, Progesterone Receptor and Human Epidermal growth factor Receptor-2 by statistics analyze. Compare the relationship between the expression of topoisomeraseⅡα with Ki67.And the relationship between the coexpression of topoisomeraseⅡα and Ki67 with the clinical and pathological characteristics.Results The results showed that the positive expression rate of TopoⅡα was 66.67%(52/78), Ki67 positive rate was 48.71%(38/78), TopoⅡα and Ki67 co-expression rate was 38.46%(30/78). There were no statistical differences between TopoⅡα and age, tumor size,ER,PR or HER-2(P>0.05).Statistically significant differences and positive correlations in TopoⅡα expression between the ones with lymph node metastases or not(P<0.05).Statistical differences and positive correlations were noted in TopoⅡα expression between Ki67(+) and Ki67(-)(P<0.05). It was showed that there is correlation between the expression of ki67 with lymph node metastasis(P<0.05). Statistically significant differences in the presence of PR(+)and PR(-) were noted in the expression rate of Ki67(P<0.05), while there was no direct relationship with age, tumor size, or expression of ER and HER-2(P>0.05).The co-expression of TopoⅡα and Ki67 were found has a correlation with tumor size(P<0.05) and lymph node metastasis(P<0.05), but no relationship were found with age, or expression of ER PR and HER-2(P>0.05). Logistic regression analysis demonstrated that the lymph node metastases or not was independent predictor both of the positive expression of TopoⅡα(P=0.007,OR =1.781,95%CI:1.175~2.700) and the co-expression of TopoⅡα and Ki67(P=0.005,OR =1.819,95%CI:1.198~2.762).It also demonstrated that the lymph node metastases(P=0.032,OR =1.730,95%CI:1.049~2.855) and the positive expression of PR(P=0.000,OR =0.126,95%CI:0.039~0.402) were independent predictors of the positive expression of Ki67.Conclusion There were statistically differences and positive correlations in TopoⅡα expression between the lymph node metastases and the expression of Ki67. There were positive relationship between the Ki67 with lymph node metastases and a negative relationship between the Ki67 with PR.The coexpression of TopoⅡα and Ki67 were related to the tumor size and lymph node metastases. Both of TopoⅡα and Ki67 can be used as proliferation markers of tumor cells, they play a significant role in the development of invasive breast cancer.The co-expression of TopoⅡα and Ki67 might provides information on the prognosis of breast cancer.